Objective To investigate the mechanism of Yangxue Antai prescription regulating autophagy and affecting placental angiogenesis in the treatment of Recurrent spontaneous abortion(RSA).Methods Rapamycin,3-methyladenine and Yangxue Antai prescription were used to intervene in pregnant mice,and then the placentas of mice were collected,and the pathological changes of pregnant mice were observed by HE staining and Masson staining;Proliferating Cell Nuclear Antigen(PCNA),Fibrinogen and CD34 protein expression were determined by immunohistochemistry;The expression of vascular endothelial growth factor(VEGF),vascular endothelial growth factor receptor 2(p-VEGFR2),p62,Beclin1,LC3Ⅰand LC3Ⅱ were determined by Western blot,and the ultrastructure of the placenta was observed by transmission electron microscopy.Results Compared with the Control group,the body weight in Rapa group decreased significantly,and the embryo absorption rate increased significantly.HE staining,Masson staining and the expression of PCNA and Fibrinogen protein showed poor placental development.The results of transmission electron microscopy(TEM)and the expression of p62,Beclin1 and LC3Ⅱ/LC3Ⅰ protein showed that the level of placental autophagy increased significantly.The expression of CD34,VEGFA and p-VEGFR2 showed abnormal placental angiogenesis.Compared with Rapa group,the body weight of mice in Rapa+Yangxue Antai group increased significantly,and the embryo absorption rate decreased significantly.HE staining,Masson staining,and the expression of PCNA and Fibrinogen protein showed that the placental development of mice improved significantly.The results of TEM and the expression of p62,Beclin1 and LC3Ⅱ/LC3Ⅰ protein showed that the level of placental autophagy decreased significantly,and the expression of CD34,VEGFA and p-VEGFR2 protein showed increased placental angiogenesis.Conclusion Yangxue Antai prescription can inhibit autophagy by inhibiting the fusion of autophagy and lysosome,improve placental development and promote placental angiogenesis in pregnant mice,and thus reduce the abortion rate.
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