Objective To determine the contents of aristolochic acid Ⅰ and aristolochic acid Ⅱ in Ditong Biyan Shui,and to provide reference for quality control.Methods A UPLC-MS/MS method was established to determine the contents of aristolochic acid Ⅰ and aristolochic acid Ⅱ.An Waters-ACQUI UPLC HSS T3 C18(2.1 mm×100 mm,1.8 μm)was used as the chromatographic column.The mobile phase was eluted with acetonitrile(A),0.1%formic acid solution(B)using a gradient elution method.The electrospray spray ion source(ESI)and positive ion multi-reaction monitoring mode were adopted.The contents were calculated using the standard curve method.Results Aristolochic acid Ⅰ was not detected in 9 samples of 17 batches of Ditong Biyan Shui(detection limit 0.13 pg),but was detected in 8 batches of samples,with the contents ranging from 0.41 to 3.60 ng·mL-1.Aristolochic acid Ⅱ was not detected in any of the samples(detection limit 0.41 pg).Conclusion A UPLC-MS/MS method has been established to determine the contents of aristolochic acid Ⅰ and aristolochic acid Ⅱ in Ditong Biyan Shui,with high specificity,high sensitivity,good repeatability,which can provide reference for quality control of aristolochic acid Ⅰ and aristolochic acid Ⅱ in Ditong Biyan Shui.
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