佛芍颗粒对新斯的明致大鼠痉挛性腹痛的作用研究
Effect of Foshao granules on spastic abdominal pain in rats
李舒冉 1郭姗姗 1包蕾 1赵荣华 1孙静 1耿子涵 1鲍岩岩 1张敬升 1徐英利 1吕耀中 2王振中 2崔晓兰 1王红梅2
作者信息
- 1. 中国中医科学院中药研究所生物安全实验室,北京 100700
- 2. 中药制药过程控制与智能制造技术全国重点实验室,江苏 连云港 222000;江苏康缘药业股份有限公司,江苏连云港 222000
- 折叠
摘要
目的 观察佛芍颗粒对新斯的明诱导痉挛性腹痛大鼠模型的药效作用.方法 6周龄SD大鼠随机分为空白对照组,模型对照组,吗丁啉对照组(6.00 mg·kg-1),四逆散对照组(1.50 g·kg-1),佛芍颗粒高、中、低剂量组(24 g生药·kg-1、12 g生药·kg-1、6 g生药·kg-1),每组10只,雌雄各半.采用腹腔注射新斯的明致大鼠痉挛性腹痛.观察大鼠疼痛潜伏期、疼痛持续时间和疼痛发生率,ELISA法检测血清P物质、胃泌素(gastrin,GT)和胃动素(motilin,MTL)含量,并观察大鼠胃组织和结肠组织的病理变化.结果 佛芍颗粒可以明显延长疼痛潜伏期(P<0.05,P<0.01)、缩短疼痛时长(P<0.01)并降低疼痛发生率,降低血清中P物质(P<0.01)、GT(P<0.01)和MTL含量(P<0.01),减轻大鼠胃和结肠的黏膜增生、间质炎症及杯状细胞肿大和增多.结论 佛芍颗粒能够改善新斯的明诱导的痉挛性腹痛.
Abstract
Objective To study the effects of Foshao granules on spastic abdominal pain in rats.Methods Six-week-old SD rats were divided into the normal control group,model group,domperidone control group(6.00 mg·kg-1),Sini powder control group(1.50 g·kg-1)and Foshao granule groups(24 g crude drug·kg-1,12 g crude drug·kg-1 and 6 g crude drug·kg-1),with 10 mice in each.Spastic abdominal pain in rats was induced by intraperitoneal injection with neostigmine.The incubation,duration and incidence of pain as well as the pathological changes in the stomach and colon of the rats were observed,while serum contents of substance P,GT and MTL were detected by ELISA.Results Foshao granules could prolong pain incubation(P<0.05,P<0.01),shorten the pain duration(P<0.01),lower the incidence of pain,reduce the serum contents of substance P(P<0.01),GT(P<0.01),MTL(P<0.01),and inhibit mucosal hyperplasia,interstitial inflammation and the enlargement and increase of goblet cells in of the stomach and colon of rats.Conclusion Foshao granules can improve neostigmine-induced spastic abdominal pain.
关键词
佛芍颗粒/新斯的明/痉挛性腹痛/药效/大鼠Key words
Foshao granules/neostigmine/spastic abdominal pain/pesticide effect/rats引用本文复制引用
基金项目
国家自然科学基金(82141206)
中央级公益性科研院所基本科研业务费专项(ZZ15-YQ-035)
中央级公益性科研院所基本科研业务费专项(ZXKT21031)
中国中医科学院科技创新项目(CI2021B015)
出版年
2024
NETL
NSTL
万方数据