Objective To predict the mechanism of roxadustat combined with Danggui Buxue Tang against renal anemia(RA)based on network pharmacology,and to verify the result via animal experiments.Methods The target proteins and signaling pathways were retrieved from PharmMapper,TCMSP,STRING,GeneCards,OMIM and DAVID database.The potential targets of roxadustat combined with Danggui Buxue Tang in treating RA were explored.A"drug-target-pathway"model was established to predict possible biological pathways.Subsequently,rat experiments were conducted to validate the main targets.Results Network pharmacology research found that the combination of roxadustat and Danggui Buxue Tang mainly exerted therapeutic effects on renal anemia through four common protein targets,namely NR3C2,AR,NR1I3,and RUNX1T1,as well as through 16 common signaling pathways such as PI3K-Akt pathway.The combination of roxadustat and Danggui Buxue Tang could upregulate the expression of target protein AR but inhibit the expression of target protein NR3C2 to exert its effects.Conclusion The combination of roxadustat and Danggui Buxue Tang can improve inflammation and oxidative stress in the body by inhibiting the expression of NR3C2 and upregulating the expression of AR,thereby exerting a therapeutic effect on renal anemia.
维普
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