Objective To investigate the effect of Lujiao formula on cardiac hypertrophy induced by angiotensin Ⅱ(Ang Ⅱ)through regulating the NF-κB signaling pathway.Methods Ang Ⅱ was used to establish a cardiac hypertrophy model of neonatal rat cardiac myocytes(NRCMs).The cells were divided into normal group,Ang Ⅱ model group,Lujiao formula+Ang Ⅱ group,BAY11-7082+Ang Ⅱ group and Lujiao formula+BAY11-7082+Ang Ⅱ group.The effect of Lujiao formula with final concentrations of 0,0.1,0.3,1,3,and 10 μ g·mL-1 on the surface area of cardiomyocytes was detected.The level of tumor necrosis factor α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6)and interleukin-2(IL-2)in culture medium of cells treated with Lujiao formula at effective concentrations of 10 and 100 μ g·mL-1 were detected by enzyme-linked immunosorbent assay(ELISA).Quantitative real-time polymerase chain reaction(RT-qPCR)was used to quantify the gene expression of ANP and BNP.Protein expression of NF-κB p65,p-NF-κB p65(Ser536),p-IKK(Ser176/180),IκBα and p-IκBα(Ser32)were detected by Western Blot.Results Compared with normal group,the surface area of cardiomyocytes in model group was significantly increased(P＜0.01 Or P＜0.05),and the mRNA level of hypertrophy markers ANP and BNP were increased(P＜0.01),and the levels of pro-inflammatory factors TNF-α,IL-1β and IL-6 were increased(P＜0.01).The level of anti-inflammatory factor IL-2 was decreased(P＜0.01),the protein expressions of p-NF-κBp65(Ser536),p-IKK(Ser176/180)and p-IκBα(Ser32)were significantly increased(P＜0.01),and the protein expressions of IκBα were decreased(P＜0.01).Compared with the model group,Lujiao formula could significantly inhibit the increase of surface area of cardiomyocytes(P＜0.01),and inhibit the expressions of ANP and BNP mRNA of cardiomyocytes(P＜0.01),and reduce the levels of TNF-α,IL-1β and IL-6 in cardiomyocyte culture medium(P＜0.01),and increase the level of IL-2(P＜0.01).Lujiao formula could significantly decrease the protein expressions of p-NF-κB p65(Ser536),p-IKK(Ser176/180)and p-IκBα(Ser32),and increase the protein expressions of IkBα(P＜0.01 or P＜0.05),and the effect of Lujiao formula on these protein levels was comparable to that of BAY11-7082(P＞0.05).Conclusion Lujiao formula could significantly inhibit cardiac hypertrophy induced by angiotensin Ⅱ through inhibiting the NF-κB signaling pathway.
Lujiao FormulaCardiomyocytesAng ⅡHeart FailureCardiac HypertrophyNF-κB Signaling PathwayInflammationSD Neonatal Rat
维普
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