Objective To establish a UPLC-MS/MS method to determine the contents of 15 components in Dandelion leaves in order to provide a reference for quality evaluation.Methods The Waters Acquity UPLC® HSS T3 Column was used while acetonitrile-0.1％formic acid water served as the mobile phase.Negative electrospray ionization was performed in a multiple reaction monitoring(MRM)mode.Cluster analysis and least partial binary analysis were conducted for evaluation.Results The 15 components had a good linear relationship within their respective range(r≥0.997 7),with good precision and accuracy(RSD＜5％).The samples could be divided into four categories by clustering and OPLS-DA analysis,and chichoric acid,cafftaric acid,rutin,esculetin,hyperoside,isoquercetin and cynaroside were used as reference for the selection of quality markers in Dandelion leaves.Conclusion This method is accurate enough to provide references for quality control of Dandelion leaves.
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