Determination of 4F-MDMB-BUTICA in Mice Plasma and Brain by UHPLC-MS/MS
Objective:To establish a liquid chromatography tandem mass spectrometry(UHPLC-MS/MS)method for the determination of 4F-MDMB-BUTICA in mice blood and brain tissues.Methods:Liquid chromatography tandem mass spectrometry was used,with a C18 reverse phase chromatographic column and 0.1%formic acid water and acetonitrile as mobile phases.The liquid-liquid extraction was selected as pre-treatment method,and 5F-MDMB-PICA was used as the internal standard substance to quantitatively detect 4F-MDMB-BUTICA in blood and brain tissue samples of mice after an abuse of 4F-MDMB-BUTICA.The methodology was validated.Results:There was a good linear relationship between 4F-MDMB-BUTICA in plasma and brain tissue in the range of 0.1-100 ng/ml(R2 was 0.9942 and 0.9939,respectively),with a minimum detection limit of 0.1 ng/ml.The intra day accuracy and inter day accuracy were both between 85%and 115%,and intra day precision and inter day precision were less than 15%.The matrix effect was in the range from 85%to 115%,indicating that there was no significant enhancement or inhibition effect in this method.Conclusion:This study established an accurate,sensitive,and reliable detection method for 4F-MDMB-BUTICA in blood and brain tissue samples using liquid chromatography tandem mass spectrometry(UHPLC-MS/MS),laying the foundation for animal related addiction research on this substance.
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