Regulatory Effect of Vitamin D and its Receptor on Inflammatory Factors in Macrophages of Oral Lichen Planus
Objective:To observe the effect of oral lichen planus (OLP) monitoring vitamin D and its receptor (VD/VDR ) on the regulation of macrophage inflammatory factors. Methods:A total of 80 patients with oral lichen planus who were treated in our hospital from August 2022 to August 2023 were included in the study group,and 80 healthy subjects were selected as the control group during the same period. The expression levels of vitamin D receptor and interleukin-10 overexpressed by THP-1 cell line and mononuclear macrophage in the two groups were monitored. The expression of inflammatory factors in macrophages was monitored after OLP was treated with vitamin D. The macrophages in OLP lesions were extracted,and the levels of inflammatory factors were detected by real-time quantitative PCR. The diagnostic accuracy of monitoring vitamin D and its receptor (VD/VDR) in the study group and the control group was analyzed. Results:Compared with the healthy control group,the expression levels of IL-10,IL-12,IL-1β,IL-6 and TNF-α in the study group were increased,and the difference was statistically significant (P<0.05). The expression of IL-10 in oral lichen planus macrophages after vitamin D treatment increased and the expression of pro-inflammatory factors decreased. Western blot showed that the expression of IL-10 in OLP lesions was positively correlated with the expression of VDR in macrophages (r=0.9631,P<0.05),and negatively correlated with IL-12,IL-1β,IL-6 and TNF-α (r=-0.8142,r=-0.9561,r=-0.9310,r=-0.9235,P<0.05). The deficiency rate of vitamin D and its receptor (VD/VDR) in the study group was higher than that in the control group (P<0.05). Conclusion:Vitamin D and its receptor signaling pathway in macrophages of oral lichen planus can regulate the transcription level of IL-10,and then affect the expression of inflammatory factors. Monitoring the level of vitamin D and its receptor in oral lichen planus has high diagnostic value.
Interleukin-10Inflammatory factorsVitamin D receptorsVitamin DOral lichen planus
万方数据
维普
