Determination of Sodium Bisulfite in Edaravone Injection by Ion Chromatography
Objective:An ion chromatography method was established to determine the content of sodium bisulfite in edaravone injec-tion.Methods:Using hydrogen peroxide solution to oxidize unstable sulfite to more stable sulfate in water,using an ion chromatograph for detection.Using 10 mmol·L-1 potassium hydroxide solution as eluent.The chromatographic column is Dionex IonPAC AS11-HC,250 mm × 4 mm,5μm.And connect a protective column with the same packing as the analysis column,with a column temperature of 25 ℃,a flow rate of 1.0 mL·min-1,and an injection volume of 20 μL.Results:Under experimental conditions,there is a good line-ar relationship within the range of 2.5 μg·mL-1 to 15 μg·mL-1,with a linear correlation coefficient of 0.999 7.The detection limit of the method is 8 ng·mL-1,the quantitative limit is 20 ng·mL-1,and the recovery rate of sample spiking is 99.29％-101.18％.Conclusion:Compared with the traditional chemical reaction colorimetry ultraviolet spectrophotometry,ion chromatography is easy to op-erate,has a low detection limit,and the sample is more stable during the detection process.The determination results are reliable and have good reproducibility,making it suitable for rapid sample detction in large quantities.
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