Objective:In response to the quality variations observed in Bombyx Batryticatus,an assessment of Bombyx Batryticatus from different origins was conducted,leading to improve the quality standard.Methods:HPLC methods were established to determine the content of beauvericin and ammonium oxalate in Bombyx Batryticatus.58 batches of representative medicinal materials and decoction pieces of Bom-byx Batryticatus from different sources were analyzed.Results:Among the 58 batches of samples,beauvericin and ammonium oxalate were not detected in 10 batches,accounting for 17.2%.The contents of beauvericin ranged from 0.010 1%to 0.119 2%,and the contents of ammonium oxalate ranged from 4.71%to 10.84%,showing a significant positive correlatation(P<0.01).Conclusion:The established methods have strong specificity,high sensitivity and good repeatability,which can effectively evaluate and control the quality of Bombyx Batryticatus.There is a high quality risk of Bombyx Batryticatus,and the quality standard needs to be further improved.