目的:建立败酱草的薄层鉴别方法(TLC)及HPLC测定东莨菪内酯含量测定方法,为败酱草的质量研究提供依据.方法:采用TLC鉴别法,HPLC作为含量测定方法,色谱柱:Ultimate Plus C18(4.6 mm ×250 mm,5µm);流动相:乙腈-0.5％冰醋酸(15:85);柱温:25℃;流速:1 mL·min-1;检测波长:343 nm.结果:薄层鉴别中检出与对照药材相同颜色的荧光斑点;东莨菪内酯在0.838 4～20.96 μg·mL-1范围内线性关系良好(函数关系为Y=39.233X+7.021 5,r=0.999 9),回收率为91.0％～97.9％,均值为95.4％,RSD(n=6)为3％.结论:本方法鉴别专属性较好,含量测定方法专属性、稳定性、重现性、准确度良好,可用于败酱草的质量控制.
Study on the Quality of Herba Patriniae
Objective:To establish a TLC method and a HPLC method for the determination of scopolamine lactone content of Herba Patriniae,provide a basis for the quality research of Herba Patriniae.Methods:Using TLC as the identification method.Using HPLC as the content determination method,the chromatographic column:Ultimate Plus C18(4.6 mm x 250 mm,5 μm).Mobile phase:acetoni-trile-0.5％glacial acetic acid(15:85).Column temperature is 25 ℃.Flow rate is 1 mL·min-1.The detection wavelength is 343 nm.Results:Fluorescent spots with the same color as the reference medicinal material were detected in TLC identification;Scopolamine lactone has a good linear relationship in the range of 0.838 4-2.96 µg·mL-1(functional relationship:Y=39.233X+7.021 5,r=0.999 9),the recovery rate is 91.0％-97.9％,the average value is 95.4％,and the RSD(n=6)is 3％.Conclusion:the identi-fication method has good specificity,the content determination method is specificity,stability,reproducibility and accuracy,which can be used for the quality control of Herba Patriniae.
万方数据
维普
