Discussion on the Determination Method and Uncertainty of Luteolin in the Current Quality Standard of Lonicerae Japonicae Flos
Objective:To explore the measurement uncertainty of the content determination method luteolin in the current quality standard of Lonicerae Japonicae Flos.Methods:The content of luteolin was determined using the method specified in the Chinese Phar-macopoeia 2020 edition for Lonicerae Japonicae Flos.The measurement uncertainty of the method was calculated and its impact on the judgment of the inspection results was evaluated.Results:The experimental results indicate that there are differences in the determination results of luteolin content in the same batch of Lonicerae Japonicae Flos samples after adjusting the mobile phase ratio in accordance with the chromatographic parameter adjustment requirements of high-performance liquid chromatography in the Chinese Pharmacopoeia 2020 edition volumeⅣ.The determination result of luteolin content in Lonicerae Japonicae Flos was(0.048±0.015)％(the confidence prob-ability was 95％,k=2).Conclusion:The measurement uncertainty results of the method indicate that the adjustment of chromatographic parameters contributes the most to the uncertainty component of this experiment.There is a significant difference in the determination results before and after the adjustment of chromatographic parameters,which affects the conformity judgment of the test results.The deter-mination method of luteolin in Lonicerae Japonicae Flos needs to be improved and revised.
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