Establishment of UPLC Fingerprint Chromatograms for JiuweiQianghuo Pills and Chemical Pattern Recognition
Objective:Toestablish UPLC(Ultra-Performance Liquid Chromatography)fingerprint chromatograms for JiuweiQianghuo Pills and conduct chemical pattern recognition.Methods:Waters Acquity UPLC HSS T3 column(2.1 mm × 100 mm,1.8 μm)was used.Gradient elution was performed with 0.1％phosphoric acid and acetonitrile.The injection volume was 1 μL,the column tempera-ture was set at 40℃,the flow rate was 0.35 mL·min-1,and detection was carried out at 254 nm.Twelve batches of JiuweiQianghuo Pills were analyzed using traditional Chinese medicine fingerprint similarity analysis software and chemical pattern recognition techniques.Results:By comparing the 12 batches of samples,24 common peaks were identified.UPLC fingerprint chromatograms of Ji-uweiQianghuo Pills were established,and 13 components were identified.The similarity values among the 12 batches of samples were all above 0.90.The 12 batches of samples were divided into 3 categories by cluster analysis and principal component analysis.The orthogonal partial least square method and discriminant analysis were used to predict that 2 of them were the key factors causing the difference be-tween batches and could be used as the quality signature components of this product.Conclusion:The established UPLC fingerprint chro-matograms for JiuweiQianghuo Pills can comprehensively evaluate the intrinsic quality of the product.Combined with chemical pattern rec-ognition analysis,they can provide reference data for setting quality standards for JiuweiQianghuo Pills.
国家科技期刊平台
NSTL
万方数据
