Mechanism of Wenyang Sanhan Decoction in Treating Stenosing Tenosynovitis Based on Network Pharmacology
Objective:To explore the therapeutic mechanism of Wenyang Sanhan Decoction on stenosing tenosynovitis using method of network pharmacology.Methods:The active components and potential target genes of Wenyang Sanhan Decoction were searched and screened by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).These genes were mapped with the tenosynovitis related genes obtained by Genecards and OMIM database.The gene names of the targets were sorted out and stand-ardized based on the Uniprot data platform,and String 11.5 database and Cytoscape 3.9.1 software were used to construct the protein-pro-tein interaction(PPI)network and the active ingredient-core target network.Then,gene ontology(GO)and Kyoto Encyclopedia of Gene and Genome(KEGG)enrichment analysis of core target genes were carried out by using DAVID 6.8 platform.Results:A total of 41 ef-fective chemical components and 96 action targets were screened out.The core active components of Wenyang Sanhan Decoction in the treatment of tenosynovitis are β-sitosterol,quercetin,kaempferol,myricetin and so on,and core target genes were mainly involved in IL6,VEGFA and so on.48 molecular functions were obtained by GO functional enrichment analysis,and 47 signal pathways were ob-tained by KEGG enrichment analysis,of which TNF signal pathway was the most closely related.Conclusion:Wenyang Sanhan Decoction can inhibit inflammation through multiple targets and pathways,so as to achieve the purpose of treating tenosynovitis.