Establishment and Analysis of Fingerprint Spectra of Compound Daqingye Mixture
Objective:Establish a fingerprint of Compound Daqingye Mixture to provide a basis for its quality control.Methods:HPLC method was used for determination,with Shim-pack GIST C18 as the chromatographic column(250 mm×4.6 mm,5 μm).The mobile phase is methanol-0.02％phosphoric acid,gradient elution,flow rate is 1.0 mL·min-1,and detection wavelengths are 260 nm and 326 nm.Results:A total of 20 common peaks were identified,and 8 components were identified through comparison with reference materials,including adenosine,gallic acid,chlorogenic acid,purple flower front saponin,aloe emodin,rhein,emodin,and chrysophanol.There are differences in the similarity between different manufacturers and different batches of formulations from the same manu-facturer.Conclusion:The HPLC fingerprint established in this study has good stability and repeatability,providing a reference for the quality control of Compound Daqingye Mixture.
Compound Daqingye MixtureFingerprintQuality control
万方数据
维普
