The Improvement of Quality Standard for Ganmao Tuire Keli
Objective:To improve the quality standard of Ganmao Tuire Keli,To ensure the stability of its efficacy and the safety of medication.Methods:The qualitative identification of Bistort Rhizome,Radix and Folium were conducted by Thin-layer chromatography(TLC).The content of uridine,guanosine and adenosine in Radix and Folium were determined by high performance liquid chromatogra-phy with Diode Array Detection(HPLC-DAD).Results:The characteristic spots in TLC were clear,with good separation and no interfer-ence in the negative control.The linear relationships for uridine,guanosine,and adenosine were good within the ranges of 0.051 4-2.055 7 µg,0.045 9-1.837 6 µg,and 0.052 5-2.101 3 µg,respectively.The average recoveries were 94.88％-105.0％with the RSD of 1.25％-1.63％.Conclusion:The established thin-layer chromatography and high-performance liquid chromatography methods are easy to operate,with good specificity and accuracy,providing a reference and basis for the further optimization and improvement of the quality standards for Ganmao Tuire Keli.
Ganmao Tuire KeliThin-Layer Chromatography(TLC)High-Performance Liquid Chromatography(HPLC)Quality standard
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