Objective To establish an efficient approach for isolating high-quality total RNA from leaves of Codonopsis pihsula. Methods Total RNA from the leaves of Codonopsis pilosida cultivated in Lingchuan GAP planting base of Shanxi China, was isolated with modified CATB method, Trizol method arid total RNA isolation kit metltod, respectively. Ultraviolet spectrophotometer analysis and gel elecirophoresis in denaturing formaldehyde a-garose gels were used to determine the isolated total RNA. Results Significant differences were noted among the RNAs isolated in various ways: total RNAs isolated by Trizol method and total RNA isolation kit method showed poor integrity and salt impurities of micromolecules, and these total RNAs may not fulfill further molecular biology study. Ultraviolet spectrophotometer analysis showed thai the total RNA by modified CTAB method had an A260/A280 value of 2,0, and A260/A230>2.0: the electrophoresis pattern showed thai the 28 S and 18 S RNA bands was clear and visible with their brightness ratios between 1.5 and 2.0; RT-PCR verified that total RNAs isolated by modified CTAB method were qualified for further molecular biology study. Conclusion The modified CTAB method was efficient for isolating the total RNA from leaves of Codonopsis pilosula because of the integrity and purity of the isolated RNAs.
NETL
NSTL
万方数据
维普
