Screening and analysis of the novel genes related to heart failure
Objective To explore the potential related genes and mechanisms of heart failure.Methods The dataset GSE18703 was downloaded from the Gene Expression Omnibus database(GEO),and Differentially Expressed Genes(DEGs)were analyzed using the"limma"package in R software.GO and KEGG pathway enrichment analysis were performed on DEGs to explore the biological functions and signaling pathways related to heart failure.A protein-protein interaction(PPI)network was constructed using Cytoscape software to identify key genes related to the pathogenesis of heart failure.The transcription levels of screened genes were verified in rat H9C2 cardiomyocytes induced by Ang Ⅱ using qPCR.The transcription factor network of key genes was analyzed through hTFtarget database to further investigate the mechanism of heart failure.Results A total of 292 DEGs were screened out,including 140 up-regulated genes and 152 down-regulated genes.GO analysis revealed that the DEGs were mainly enriched in extracellular regions,extracellular spaces,blood particles and other cellular components,as well as pathways such as olfactory transduction,vascular smooth muscle contraction,and metabolic pathways.KEGG pathway analysis found that DEGs were mainly enriched in cGMP-PKG signaling pathway,hypertrophic cardiomyopathy,dilated cardiomyopathy and other signaling pathways.Through the PPI network,six key genes were identified,namely Gprc6a,C3,Anxa1,Oxt,Gpr4 and Avpr1b.And 528 transcription factors were found to regulate the transcription of these six key genes.The qPCR results showed a significant difference in mRNA expression between Anxa1 and Gpr4 in heart failure,further confirming the above screening results.Conclusion Through bioinformatics analysis,we successfully identified six key genes associated with heart failure,which can help to better understand the mechanism of heart failure and explore novel therapeutic targets.
万方数据
维普
