Effects of naringin on MAPK signaling pathway in promoting osteogenic differentiation of rat bone mar-row mesenchymal stem cells
Objective To explore the impact of naringin on enhancing the osteogenic differentiation and bone segment migration repair capability of bone marrow mesenchymal stem cells(MSCs)following the principles of kidney tonification and blood circulation promotion.Methods A total of 64 six-month-old rats undergoing bi-lateral ovariectomy,unilateral tibial plateau osteotomy was performed 12 weeks postoperatively.After euthanasia,bilateral femurs and tibias of the rats were collected under sterile conditions for MSCs culture.On the 1st,2nd,3rd,5th,7th,9th,and 11th day of cultivation,the viability of MSCs was measured using MTT method and growth curves were plotted to explore the proliferation ability of rat MSCs.The surface labeled antigens CD29,CD44,and CD45 were detected using flow cytometry.The optical density(A)values of the third-generation MSCs(in good growth status)after intervention with naringin(at concentrations of 10 mmol/L,10 mmol/L,10-6 mmol/L,10-5 mmol/L,and 10-4 mmol/L)were detected using the MTT assay.The optimal concentration promoting proliferation was screened to confirm the effect of naringin in inducing MSC differentiation into osteoblasts.Results The A value was measured by MTT method,and the growth curve formed an S-shape.MSCs showed logarithmic growth after 3 days of cultivation,and entered the plateau phase after 9 days.The proliferative effects of different concen-trations of naringin on MSCs and the expression rate of ALP-positive cells were compared,and the differences were statistically significant(P＜0.05).Conclusion Naringin can promote the differentiation and proliferation of MSCs in experimental rats,increase the expression of osteocalcin,and improve the symptoms of osteoporosis in ovariectomized rats.
NaringingStem cellsMitogen-activated protein kinaseOsteocalcinCell differentiation
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