Expression of lncRNA DLGAP1-AS5 in breast cancer tissue and its effect on proliferation and invasion of breast cancer cells
Objective To explore the effect of long non-coding RNA(lncRNA)DLGAP1-AS5 on the prolif-eration and invasion of breast cancer cells and its molecular mechanism.Methods The TCGA database was used to analyze the expression level of DLGAP1-AS5 in breast cancer tissues and normal tissues,and to observe the re-lationship between the expression level of DLGAP1-AS5 and the survival period of breast cancer patients.The ex-pression level of DLGAP1-AS5 in four breast cancer cell lines:immortalized breast epithelial cells(MCF-10A),human breast cancer cells(MCF-7,HCC-1937),human breast adenocarcinoma cells(SK-BR-3),and human breast tube cancer cells(BT-549)was detected by qRT PCR.The DLGAP1-AS5 overexpression plasmid or negative con-trol plasmid was transfected into MCF-7 cells,which were designated as DLGAP1-AS5 group and NC group,re-spectively.The proliferation and invasion of MCF-7 cells were detected by MTT and Transwell assays.The target-ing relationship between DLGAP1-AS5 and miR-362-5p was detected by starBase v3.0 online software and dual luciferase reporter assay.The expression level of miR-362-5p in MCF-7 cells was detected via qRT-PCR.Addi-tionally,the proteins expression of the p38-MAPK signaling pathway in MCF-7 cells was detected by western blotting.Results Compared with normal tissues,the expression level of DLGAP1-AS5 in breast cancer tissues was lower(P＜0.01).Breast cancer patients with lower DLGAP1-AS5 expression demonstrated significantly longer overall survival and disease-free survival compared to those with higer DLGAP1-AS5 expression level(P＜0.01).When compared to MCF-10A,the expression of DLGAP1-AS5 was notably downregulated in breast cancer cell lines(P＜0.01).Among these cell lines,the reduction in DLGAP1-AS5 expression was most pronounced in MCF-7 cells(P＜0.01).Compared with the NC group,overexpression of DLGAP1-AS5 weakened the proliferation ability of MCF-7 cells(P＜0.05)and decreased their invasive ability(P＜0.01).DLGAP1-AS5 could target and bind miR-362-5p(P＜0.01).Compared with the NC group,overexpression of DLGAP1-AS5 led to a significant reduction in miR-362-5p expression(P＜0.01)and a notable downregulation of p38-MAPK signaling pathway proteins in MCF-7 cells(P＜0.01).Conclusion DLGAP1-AS5 is expressed at low levels in breast cancer tissues and cell lines,and its expression level is closely associated with the survival period of breast cancer patients.Overexpression of DLGAP1-AS5 can effectively inhibit the proliferation and invasion of breast cancer MCF-7 cells by downregulating the expression of miR-362-5p.
Breast neoplasmsRNA,long noncodingCell proliferationCell invasion
万方数据
维普
