Effect of dexmedetomidine on PPARγ/NF-κB activity in rats with sepsis under liver damage
Objective To evaluate the effect of Dexmedetomidine on PPARγ/NF-κB activity in rats with sepsis under l iver damage.Methods A total of 30 SPF-grade adult male SD rats,weighing 230-250 g,were randomly divided into sham operation group(Sham group),model group(M group),and dexmedetomidine group(D group)according to the random number table method.In addition to the Sham group,the sepsis model was estab-lished by cecal ligation and puncture method.The Sham group only exposed the cecum through laparotomy with-out ligation,and then closed the abdomen.The rats in D group were intravenously injected with 5 μg/kg dexmedetomidine through the external jugular vein catheter,which was completed within 10 minutes,followed by maintenance dosing at 5 μg·kg-1·h-1.The Sham group and M group were given an equal dose of normal saline,and all groups stopped medication before skin suturing.At 24 hours postoperatively,blood samples were collected to measure the concentrations of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),and bilirubin(BIL).The concentrations of interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)in serum were de-tected by enzyme-linked immunosorbent assay(ELISA).The histopathological results of liver tissue were observed and scored using HE staining.The expression of PPARγ,nuclear NF-κB,and IκB(inhibitor of NF-κB)proteins in liver tissue was detected by Western blot.Results Compared with the Sham group,the concentrations of serum ALT,AST,BIL,IL-6,and TNF-α were increased,the liver tissue injury score was elevated,the protein expression of PPARγ and IκB in the liver was downregulated,and the expression of NF-κB in the liver was upregulated in the other groups(P＜0.05).Compared with the M group,the concentrations of serum ALT,AST,BIL,IL-6,and TNF-α were decreased,the liver tissue injury score was reduced,the protein expression of PPARγ and IκB in the liver was upregulated,and the expression of NF-κB in the liver was downregulated in the D group(P＜0.05).Conclusion Dexmedetomidine can alleviate liver injury in septic rats,and its mechanism is related to reducing inflammatory responses through the PPARγ/NF-κB signaling pathway.
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