4-phenylbutyric acid alleviates endoplasmic reticulum stress in trophoblasts through NF-κB pathway
Objective To explore the signaling pathway of 4-phenylbutyric acid(4-PBA)in alleviating endoplasmic reticulum stress.Methods A endoplasmic reticulum stress model was established using the immortalized trophoblast cell line HTR-8/Svneo.After adding 4-PBA for culture,the expression of endoplasmic reticulum stress specific molecules GRP78,PERK,and CHOP were compared using Western blot method.The same method was then used to detect the expression of NF-κB,caspase-3,and Bcl-2.Results As the concentration of 4-PBA increases,the expression level of endoplasmic reticulum stress characteristic proteins decreased,and the differences in expression levels between different concentrations were statistically significant.After culturing with different concentrations of 4-PBA added to the medium,the expression level of NF-κB decreased with the increase of 4-PBA concentration,and the difference was statistically significant.Conclusion 4-PBA alleviates endoplasmic reticulum stress in trophoblasts through the NF-κB pathway
万方数据
维普
