Therapeutic effect of Jiawei Simiao Powder on acute gouty arthritis rats and its influence on the expres-sion of P2X7R and NLRP3 protein in synovial tissue
Objective To study the therapeutic effect of Jiawei Simiao Powder on acute gouty arthritis rats and its influence on the expression of P2X7R and NLRP3 protein in synovial tissue.Methods A total of 60 male SD rats were divided into 6 groups using the random number table method,with 10 rats in each group.The rat model with hyperuricemia and acute gouty arthritis was replicated.The normal control group was given an equal volume of physiological saline in the corresponding parts.While the Jiawei Simiao Powder group was given a low,medium or high dose of water extract(4.24 g/kg、8.48 g/kg、16.96 g/kg).The colchicine group was given colchicine suspension(3 mg/kg).The model group was adminstered an equal volume of 0.9％sodium chloride injection via gavage.After 4 h,8 h,12 h,24 h and 48 h treatment,the swelling of the right ankle joint and the gait of the rats were observed.Two hours after the last administration,blood samples were collected from the abdominal aorta,serum uric acid concentration,liver and kidney function and serum ATP level were detected by biochemical method,serum IL-1β and IL8 levels were determined by enzyme-linked immunosorbent assay(ELISA),and ankle synovium tissues were collected.Hematoxylin-eosinstaining(HE)was used to observe the pathology of synovial tis-sue,and the expression of NLRP3 and P2X7R proteins in synovial tissue was detected by Western Blot(WB).Results Except for the normal control group,the ankle joint swelling rate of rats in each group began to increase from 4-8 h after modeling and peaked at 24 h before decreasing.After 48 hours of modeling,the swelling rates of the colchicine group and the low,medium,and high-dose groups of Jiawei Simiao powder were significantly re-duced,and the gait was gradually improved(P＜0.05).Compared with the blank group,the synovial tissue in the model group showed obvious pathological changes of arthritis,and the synovial tissue was exfoliated,necrosis,and synovial cell proliferation were observed under the microscope.The levels of serum ATP,IL-1β,blood uric acid and IL-8 in the low,medium and high dose groups of supplemented Simiao Powder and colchicine group were sig-nificantly reduced,and the pathological condition of synovial arthritis was improved.The protein expressions of P2X7R and NLRP3 in synovial tissue were significantly decreased(P＜0.05).Compared with colchicine group,supplemented Simiao powder had less damage to liver and kidney function(P＜0.05).Conclusion Jiawei Simiao Powder can reduce the blood uric acid level of hyperuricemia and gout model rats,reduce the damage to liver and kidney function,inhibit the expression of NLRP3 and P2X7R proteins in ankle synovium tissue of rats with acute gouty arthritis,and reduce local tissue inflammatory response,and thus exert therapeutic effects.
Jiawei Simiao PowderHyperuricemiaArthritis,goutyP2X7R and NLRP 3 proteins
万方数据
维普
