Comprehensive evaluation of Xieqing Pills by HPLC multi-component quantitative combined with chemo-metrics and grey relational analysis
Objective To establish a method for quantifying the multiple indicator components of Xieqing Pills,and to evaluate its comprehensive quality by chemometrics and grey relational analysis GRA methods.Methods Determined by HPLC,chromatographic column was Venusil MP C18 column,and acetonitrile-0.2%glacial acetic acid as the mobile phase.The contents of swertiamain,gentiopicrin,sweroside,shanzhiside,garypto-side,genipin 1-gentiobioside,geniposide,prim-O-glucosylcimifugin,cimifugin,sec-o-glucosylhamaudol,senkyuno-lide H,senkyunolide I,senkyunolide A and ligustilide of Xieqing Pills were determined.Chemometrics was used to explore differential markers affecting the product quality of Xieqing Pills.The relative correlation degree was used as the measure to analyze the comprehensive quality of Xieqing Pills.Results The methodological validation of the content determination of 14 components was completed,and the results met the requirements of Chinese Pharma-copoeia.The chemometric analysis showed that geniposide,gentiopicrin,ligustilide,genipin 1-gentiobioside,senkyunolide A,prim-O-glucosylcimifugin and gardenoside were the main potential markers affecting the quality of Xieqing Pills.The results of GRA showed that the relative correlation degree was between 0.385 and 0.584,which could be used to evaluate the quality of Xieqing Pills.Conclusion The quality of Xieqing Pills can be compre-hensively evaluated by combining HPLC multi-component quantification with chemometrics and GRA method.
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