Simultaneous determination of 7 components in Dengqi Tongmai Granules by HPLC
Objective To determine the content of effective components in Dengqi Tongmai Granules was determined by high performance liquid chromatography(HPLC),and its main chemical components were analyzed.Methods Using a Diamonsil C18 chromatographic column(4.6 mm×250 mm,5 μm),with water(containing 0.1％phosphoric acid)-acetonitrile as the mobile phase and gradient elution,the detection wavelength was set at 283 nm.The content of effective components in three batches of Dengqi Tongmai Granules was determined,and the main chemical components of the samples were analyzed.Results The method can be used to determine 7 active com-ponents in Dengqi Tongmai Granules,including scutellarin,naringin and neohesperidin,and so on.The results of the main chemical components analysis showed that the contents of top scutellarin,neohesperidin and naringin were the highest,which were 1.52 mg/g,2.54 mg/g and 2.46 mg/g,respectively.Conclusion This method has good practicability and feasibility,providing a scientific basis for the quality control and clinical application of Dengqi Tongmai Granules.
Deng Qi Tong Mai GranuleChromatography,liquidQuantitative analysis of contentChemical components
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