Tetracycline resistance and 16S rRNA gene nmutation analysis of clinical strains of Helicobacter pylori
Objective To explore tetracycline resistance of Helicobacter pylori and analyze the mutation sites of resistance genes in tetracycline-resistant strains,and to provide guidance for selecting sensitive antibiotics for the clinical eradication of Helicobacter pylori and offer personalized treatment options for patients.Methods Helicobacter pylori was isolated and cultured from gastric mucosal tissues.The agar dilution method was used to test the tetracycline drug sensitivity of Helicobacter pylori.Polymerase chain reaction was used to amplify the 16S rRNA gene of tetracycline-resistant strains,followed by sequencing and analysis of gene mutation sites.Results A total of 82 clinical strains of Helicobacter pylori were successfully isolated,including 5 tetracycline resistant strains with a resistance rate of 6％.The tetracycline resistance gene in the study region was characterized by a single-base mutation of AGA926-928CGA in Helicobacter pylori.Additionally,two resistant strains showed mu-tations of G970A and A1119G,respectively.Conclusion The clinical strains of Helicobacter pylori in this region have a low resistance rate to tetracycline.The A926C mutation in the 16S rRNA gene is associated with Heli-cobacter pylori resistance to tetracycline.
Helicobacter pyloriIsolation and cultivationTetracyclineDrug resistance16S rRNA
NETL
NSTL
万方数据
