Effects of Eriocitrin on oxidative stress and inflammatory response in carotid atherosclerosis rats by reg-ulating Nrf2/NQO-1/HO-1/NF-κB signaling pathway
Objective To explore the effects of Eriocitrin on oxidative stress and inflammatory response in carotid atherosclerosis(CAS)rats,and to explore its mechanism.Methods Rats were randomly divided into a control group,a model group,a low-dose Eriocitrin group,a medium-dose Eriocitrin group,a high-dose Eriocitrin group,and a high-dose Eriocitrin+Brusatol group,with 10 rats in each group.CAS rat model was constructed,and blood lipid indicators such as triglyceride(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)were detected,hematoxylin-eosin(HE)staining was applied to observe pathological changes in carotid artery tissue,oil red O staining was applied to observe the lipid accumula-tion in carotid tissue,kits were applied to detect oxidative stress indicators such as malondialdehyde(MDA),su-peroxide dismutase(SOD),and catalase(CAT),enzyme-linked immunosorbent assay kit was applied to detect lev-els of reactive oxygen species(ROS)and inflammatory cytokines such as interleukin-1β(IL-1β),IL-10,and tumor necrosis factor-α(TNF-α),and Western blot was applied to detect the expression of nuclear factor E2 related fac-tor 2(Nrf2)/quinone oxidoreductase 1(NQO1)/heme oxygenase 1(HO-1)/nuclear factor κB(NF-κB)signaling path-way proteins.Results Compared with the control group,the carotid artery tissue intima of rats in the model group was obviously thickened,the AS plaques and lipid deposition were increased,the TG,TC,LDL-C,proportion of lipid accumulation,IL-1 β,TNF-α,MDA,ROS,p-NF-κB p65/NF-κB p65 levels were obviously increased,and the HDL-C,IL-10,SOD,CAT,p-Nrf2/Nrf2,NQO-1,HO-1 levels were obviously decreased(P＜0.05);compared with the model group,the carotid artery tissue intima of the low,medium,and high dose Eriocitrin groups was relatively intact,the AS plaques and lipid deposition was decreased,the TG,TC,LDL-C,proportion of lipid accumulation,IL-1β,TNF-α,MDA,ROS,p-NF-κB p65/NF-κB p65 levels were obviously decreased,and the HDL-C,IL-10,SOD,CAT,p-Nrf2/Nrf2,NQO-1,HO-1 levels were obviously increased(P＜0.05);Brusatol reversed the improve-ment effect of Eriocitrin on oxida-tive stress and inflammatory response in CAS rats(P＜0.05).Conclusion Erioc-itrin can regulate the level of blood lipids,as well as inhibit inflammatory response and oxidative stress in CAS rats,and its mechanism of action may be related to the regulation of Nrf2/NQO-1/HO-1/NF-κB signaling pathway.
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