Early diagnostic value of TGF-β1 related urinary exosome miRNAs in patients with type 2 diabetic kidney disease
Objective To explore the clinical value of transforming growth factor-β,(TGF-β1)-related serum microRNA in the early diagnosis of diabetes nephropathy(DKD).Methods A total of 260 patients of type 2 diabetes mellitus(T2DM)treated in Huzhen Town Health Center and Jinyun County People's Hospital from January 2019 to December 2021 were collected as the study cohort.According to the urinary albumin excretion rate(UAER),the study cohort was divided into a simple T2DM group(135 cases),an early DKD group(82 cases),and a clinical DKD group(43 cases).Another 50 healthy volunteers were selected as the control group(NC).The lev-els of serum miR-27a,miR-27b,miR-29c,miR-200c,and TGF-β1were detected in each group,and correlation analysis was conducted between the indicators.The early diagnostic value of serum miRNAs for DKD was ana-lyzed by receiver operating characteristic(ROC)curve.Results The serum levels of miR-27a,miR-27b,miR-200c and TGF-β1in the simple T2DM group,early DKD group and clinical stage DKD group were higher than those in the control group,while the level of miR-29c was lower than that in the control group(F=235.624,70.351,108.127,672.304,all P＜0.001).TGF-β1 was positively corr-elated with serum miR-27a,miR-27b,and miR-200c,but negatively correlated with miR-29c(r=0.668,0.429,0.618,-0.502,all P＜0.001).The areas under the diagnostic curve of serum miR-27a,miR-27b,miR-29c and miR-200c for DKD in T2DM patients were 0.913,0.775,0.812 and 0.894,and the sensitivity and specificity of miR-27a and miR-200c were both greater than 0.7.In the validation cohort,the diagnostic AUCs of serum miR-27a,miR-27b,miR-29c,and miR-200c for DKD in T2DM patients were 0.901,0.787,0.802,and 0.896 respectively,and the differences were statistically significant(Z=-9.012,P＜0.01).Conclusion Serum miR-27a,miR-27b,miR-29c,and miR-200c have certain diagnostic val-ue for early diabetic nephropathy,and their mechanism of action may be related to the regulation of TGF-βi sig-naling pathway.
Diabetic nephropathiesMicrouria protein excretion rateMicroRNATransforming growth factor beta 1Early diagnosis
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