Resveratrol inhibits proliferation,migration and promotes apoptosis of human colon cancer SW480 cells via PI3K/Akt/mTOR pathway
Objective To investigate the inhibitory effect of resveratrol on proliferation,migration and apoptosis of human colon cancer cell line SW480,and to analyze the regulatory effect of resveratrol on the PI3K/Akt/mTOR pathway.Methods Human colon cancer cell line SW480 was cultured,passaged,and grouped into three groups at the logarithmic phase.The blank control group(BC group),positive control group(PC group),and low/medium/high dose resveratrol group(LD/MD/HD group)were set up with three compound holes.Resveratrol LD,MD and HD groups were given 50,100 and 200 μmol/L of resveratrol intervention.The PC group was given 12.5 mg/L of oxaliplatin intervention,and the BC group was given the same amount of sterile saline intervention.All of these groups were then cultured for 48 hours.The morphological changes of the cells were observed by light microscopy.Cell proliferation was detected by MMT assay.Cell migration was detected by Transwell chamber mi-gration test.Cell apoptosis was detected by flow cytometry.The relative expression of PI3K,Akt,and mTOR was detected by RT-qPCR.The relative expression of PI3K,Akt,mTOR,P21,CyclinD1,and the phosphorylation of Akt and mTOR were detected by Western blot.Results There were no obvious changes in cell morphology in the BC group.Cell shrinkage,roundness,volume reduction,refraction,and adherence weakening,cell exfoliation were observed in the other four groups.A few fragments were observed in the PC group,obvious fragments in the MD group,and more fragments in the HD group.The inhibition rate of proliferation in the HD group was the highest,followed by the MD group,the PC group was slightly lower and the LD group was lower.The relative width of scratch area was the widest in the BC group,followed by the LD group,slightly narrower in the PC group,narrow-er in the MD group and narrowest in the HD group.The apoptotic rate of the HD group was the highest,followed by the MD group;the PC group was slightly lower,the LD group was lower,and the BC group was the lowest.The relative expression of P13K and protein,P21,CyclinD1 protein,p-Akt,p-mTOR in the BC group were the highest,followed by the LD group,the PC group were slightly lower,the MD group were lower,the HD group were the lowest.The relative expression of P21 protein in the HD group was the highest,followed by the MD group,the PC group was slightly lower,the LD group was lower,the BC group was the lowest.There were significant differences in the above indicators between the two groups(P＜0.05).Conclusion Resveratrol can promote the morphological changes of human colon cancer cell SW480,inhibit its proliferation and migration,and promote its apoptosis.It is speculated that it may be achieved by regulating PI3K/AKT/mTOR pathway,up-regulating PI3K gene and protein expression,increasing phosphorylation level of Akt and mTOR,enhancing the downstream P21 protein expression and inhibiting downstream CyclinD1 protein expression.
万方数据
维普
