Effects of resveratrol on proliferation and apoptosis of colon cancer cells by regulating PI3K/Akt pathway
Objective To explore the effects of resveratrol on the proliferation and apoptosis of colon cancer cells by regulating the PI3K/Akt pathway.Methods Human colon cancer SW480 cells were cultured in vitro and the proliferative capacity was evaluated using the MTT assay.The cells were divided into a control group,a low-dose resveratrol group(20 μmol/L),a medium dose resveratrol group(40 μmol/L),and a high-dose resveratrol group(80 μmol/L).The migration abilities of the cells were assessed by the scratch assay,while their invasion capabilities were determined using a Transwell chamber.Apoptotic responses were quantified through flow cytometry and acridine orange staining.Furthermore,the mRNA expression levels of Bcl-2,Bax,and Cas-pase-3 were analyzed by RT-PCR.Additionally,the activity protein of the PI3K-Akt signaling pathway was in-vesti-gated in each group utilizing the Western.Results Compared with the control group,20,40,and 80 μmol/L resveratrol significantly reduced cell survival rate(P＜0.05),and 48 h of culture at different concentrations of resveratrol was most suitable.Moreover,the cell migration ability was significantly weakened in different doses of resveratrol groups(P＜0.05),and the cell migration rate gradually decreased with the increase of resveratrol con-centration.On the basis of the control group,the experimental group cells treated with resveratrol showed a sig-nifi-cant increase in apoptosis rate(P＜0.05).In addition,in this study,we observed a positive correlation between cell apoptosis rate and resveratrol concentration,and the cell apoptosis rate gradually increased with the increase of resveratrol concentration(P＜0.05).Compared with the control group,the experimental group cells showed a sig-nifi-cant increase in autophagy after treatment with resveratrol,and the autophagy quantity gradually increased with the increase of resveratrol concentration(P＜0.05).Compared with the control group,human colon cancer SW480 cells treated with different doses of resveratrol showed a significant decrease in Bcl-2 level,while a signifi-cant in-crease in Bax and caspase-3 levels.Compared with the control group,the various doses of resveratrol sig-nificantly decreased P13K and p-Akt protein levels.However,there was no significant difference in Akt protein between dif-ferent doses of resveratrol groups(P＞0.05).Conclusion Resveratrol can promote apoptosis of colon cancer cells by mediating the PI3K/Akt signaling pathway,reduce their prolifera-tion ability,weaken cell migra-tion and inva-sion ability,and control the invasion and deterioration of colon cancer.
ResveratrolColonic neoplasmsPhosphatidylinositol 3-kinasesProto-oncogene proteins c-aktMolecular mechanisms of pharmacological action
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