Infection of mycoplasma pneumoniae infection in children in a certain hospital and the correlation be-tween 23S rRNA gene-site mutations and antibiotic resistance
Objective To analyze infection of mycoplasma pneumoniae infection in children in a certain hospital and the correlation between 23S rRNA gene site mutations and antibiotic resistance.Methods A total of 100 children with suspected mycoplasma pneumoniae pneumonia were selected from February 2021 to January 2023 in Changxing County Hospital of Traditional Chinese Medicine compared the infection of mycoplasma pneu-moniae in children of different genders and ages.According to the results of genetic testing,the children were di-vided into mutant group and non-mutant group,and the clinical data of the two groups were compared,and the correlation between multiple factors and drug-resistant mutations was analyzed by Logistic regression.Results Among the 100 suspected children with mycoplasma pneumoniae pneumonia,92 cases(92.0％)were positive by PCR.Among these positive cases,36 cases(39.1％)showed mutations in the 23S rRNA gene locus,while 56 cas-es(60.9％)did not show mutations.The positive rate of mycoplasma pneumoniae in female children(93.2％)was slightly higher than that in male children(91.1％),and the mutation rate of 23S rRNA gene locus(41.5％)was slightly higher than that in male children(37.2％)(P＞0.05).Children with mycoplasma pneumoniae over 3 years old not only had a significantly higher positive rate(96.8％)compared to those aged 1 to 3 years old(83.8％),but also showed a significantly higher mutation rate(47.5％)in the 23S rRNA gene locus compared to those in the 1 to 3 year old age group(22.6％)(P＜0.05).There were statistically significant differences(P＜0.05)in white blood cell count,neutrophil percentage,procalcitonin,erythrocyte sedimentation rate,duration of fever,duration of cough,length of hospital stay,duration of macrolide use during this illness,and mycoplasma pneumoniae-DNA load during this illness between the mutant group and the non-mutant group.Multivariate Logistic regression analysis revealed that there was a correlation between mycoplasma pneumoniae-DNA load and drug-resistant mutations dur-ing this illness(P＜0.05).Conclusion The risk of mutation at the 23S rRNA gene locus of mycoplasma pneumo-niae gradually increases with the age of children with mycoplasma pneumoniae pneumonia.Furthermore,there is a significant correlation between the mycoplasma pneumoniae-DNA load during this illness and the drug-resistant mutations at positions A2063G and A2064G in the V region.
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