Effects of lidocaine on p2x7-p38-IL-Iβ pathway in rats with trigeminal neuralgia
Objective To investigate the effects of lidocaine on p2x7-p38-IL-1β pathway in trigeminal neuralgia. Methods All male SD rats were divided into three groups by random number table method:sham surgery group (Sham group),surgery group (ION-CCI group),and lidocaine group (ION-CCI+L group). Sham group:blunt separation of the right infraorbital nerve without ligation+intraperitoneal injection of equal volume 0.9％sodium chloride injection;ION-CCI group:blunt separation of the right infraorbital nerve and ligation+intraperitoneal injection of equal volume 0.9％sodium chloride injection;ION-CCI+L group:blunt separation of the right infraorbital nerve and ligation+intraperitoneal injection of lidocaine 10 mg·kg-1·d-1. Mechanical pain threshold measurements were performed on rats before surgery and on postoperative days 1,3,5,7,9,11,and 14 days after surgery,intraperitoneal injection of pentobarbital sodium (60 mg/kg) was administered for anesthesia,and brain tissue and trigeminal ganglia were collected by perfusion. The expression levels of IL-1β in the thalamus and trigeminal ganglia of rats were detected using enzyme-linked immunosorbent assay (ELISA). The expression levels of p2x7,p38,p-p38,and IL-1β in the thalamus of rats were detected using protein immunoblotting. The expression level of p-p38 in rat thalamus was detected by immunofluorescence. Results The pain threshold of ION-CCI+L group rats was lower than that of Sham group (P<0.01),but higher than that of ION-CCI group (P<0.01);The ELISA results showed that the concentration of IL-1β in the thalamus and trigeminal ganglia of rats in the ION-CCI+L group was lower than that in the ION-CCI group (P<0.01),but higher than that in the Sham group (P<0.01). The results of protein immunoblotting showed that the expression levels of p2x7 and p-p38 in the thalamus of rats in the ION-CCI+L group were lower than those in the ION-CCI group (P<0.01),but higher than those in the Sham group (P<0.01);The expression level of IL-1β in the thalamus of ION-CCI+L group rats was lower than that of ION-CCI group (P<0.01),but higher than that of Sham group (P<0.01). There was no statistically significant difference in the expression level of p38 in the thalamus of rats in all the groups ( P>0.05). The immunofluorescence results showed that the fluorescence intensity of p-p38 in the thalamic region of ION-CCI+L group rats was lower than that of ION-CCI group (P>0.05),but higher than that of Sham group (P>0.05). Conclusion Lidocaine can alleviate trigeminal neuralgia,reduce the expression level of IL-1β in thalamus and trigeminal ganglion of trigeminal neuralgia rats,and decrease the expression level of p2X7 and p-p38 in thalamus of trigeminal neuralgia rats,suggesting that lidocaine may inhibit the central nervous system inflammatory by inhibiting the p2x7-p38-IL-1β pathway,thereby alleviating trigeminal neuralgia
Receptors,purinergic p2x7MAP kinase signaling systemp38 mitogen-activated protein kinasesInterleukin-1 betaLidocaineTrigeminal neuralgia
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