Objective To investigate the expression of miRNAs in hepatocellular carcinoma (HCC) and their effects on cell proliferation,so as to screen out novel HCC-specific therapeutic targets and provide a theoreti-cal basis for the development of novel therapeutic drugs for HCC.Methods Differential expression analysis of miRNA expression data of HCC tissue samples in TCGA database was performed by edgeR,and clinicopathologi-cal parameter analysis and target prediction were also performed.PCR were performed to detect the expression of miR-139 in the tumors and paracancerous tissues of HCC patients,and then the proliferative activities of hepato-cellular carcinoma cells in the four groups of miR-139-5p mimic,mimic NC,miR-139-5p inhibitor,and inhibitor NC were detected by CCK8.Results The expression of the target gene CTAG2 was negatively correlated with the expression of miR-139-3p and miR-139-5p (P<0.05).miR-139 was correlated with Grade hypo-differentiation (P<0.001),recurrence of tumors after the initial treatment (P<0.05),T-stage Ⅱ～Ⅳ(P<0.01) and Stage Ⅱ～Ⅳ(P<0.01) were significantly negatively correlated.PCR assay,the results showed that the expression of miR-139-5p was sig-nificantly down-regulated in tumor tissues compared with paracancerous tissues.Compared with the mimic NC,miR-139-5p inhibitor,and inhibitor NC groups,miR-139-5p expression in the miR-139-5p mimic group was signif-icantly increased (P<0.05);the cell proliferation activity of the miR-139-5p mimic group was proved to be signifi-cantly lower than that of the remaining three groups by CCK8 assay.Conclusion The low expression of miR-139 and the expression of the target gene CTAG2 are both associated with poor prognosis in HCC,and the high ex-pression of miR-139-5p has an inhibitory effect on the proliferation of hepatocellular carcinoma cells.
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