骨髓细胞形态学、免疫组化、PCR诊断淋巴瘤骨髓侵犯的价值

扫码查看

原文链接

国家科技期刊平台
NETL
NSTL
万方数据

中文摘要：目的:探讨骨髓细胞形态学、免疫组化、聚合酶链反应(PCR)诊断淋巴瘤骨髓侵犯的价值.方法:选取赣州市肿瘤医院 2021 年 1 月—2023 年 8 月收治的 81 例淋巴瘤患者,均接受骨髓细胞形态学、骨髓涂片免疫组化、PCR检测,分析三者单独及联合检出骨髓侵犯的价值.结果:81 例患者中,骨髓活检检出 21 例、骨髓细胞形态学检出 32 例、骨髓涂片免疫组化检出 36 例,PCR检出 19 例.其中,联合检测与骨髓活检的一致性最好,Kappa值为 0.875;以骨髓活检为参考标准,骨髓细胞形态学、骨髓涂片免疫组化、PCR及联合检测的敏感度分别为 80.95%、90.48%、71.43%、95.24%,特异度分别为75.00%、71.67%、93.33%、95.00%,准确率分别为 76.54%、76.54%、87.65%、95.06%,阳性预测值分别为 53.13%,52.78%、78.95%、86.96%,阴性预测值分别为 91.84%、95.56%、90.32%、98.28%.联合检测的敏感度高于PCR单项检测,特异度、准确率、阳性预测值均高于骨髓细胞形态学、骨髓涂片免疫组化单项检测(P<0.05).结论:淋巴瘤患者骨髓细胞形态学检查联合骨髓涂片免疫组化、PCR检查可明显提高对骨髓侵犯的诊断效能.

外文标题：Value of Bone Marrow Cell Morphology,Immunohistochemistry and PCR in Diagnosing Lymphoma with Bone Marrow Involvement

外文摘要：Objective:To explore the value of bone marrow cell morphology,immunohistochemistry and polymerase chain reaction(PCR)in the diagnosis of lymphoma with bone marrow involvement.Method:A total of 81 patients with lymphoma in Ganzhou Cancer Hospital were selected from January 2021 to August 2023.All patients underwent bone marrow cell morphology,bone marrow smear immunohistochemistry and PCR detection,and the value of the three alone and combined detection on bone marrow involvement were analyzed.Result:Among 81 patients,21 cases were detected by bone marrow biopsy,32 cases were detected by bone marrow cell morphology,36 cases were detected by bone marrow smear immunohistochemistry and 19 cases were detected by PCR.The consistency between combined detection and bone marrow biopsy was the best,with Kappa value of 0.875.Taking bone marrow biopsy as the reference standard,the sensitivities of bone marrow cell morphology detection,bone marrow smear immunohistochemistry,PCR and combined detection were 80.95%,90.48%,71.43%and 95.24%,the specificities were 75.00%,71.67%,93.33%and 95.00%,accuracy rates were 76.54%,76.54%,87.65%and 95.06%,the positive predictive values were 53.13%,52.78%,78.95%and 86.96%,and the negative predictive values were 91.84%,95.56%,90.32%and 98.28%respectively.The sensitivity of combined detection was higher than that of PCR single detection,and the specificity,accuracy rate and positive predictive value were higher than those of bone marrow cell morphology and bone marrow smear immunohistochemistry(P<0.05).Conclusion:Bone marrow cell morphology examination combined with bone marrow smear immunohistochemistry and PCR can significantly improve the diagnostic efficiency on bone marrow involvement in patients with lymphoma.

外文关键词：

LymphomaBone marrow involvementBone marrow cell morphologyImmunohistochemistryPolymerase chain reaction

作者：

廖伟莲

展开 >

作者单位：

赣州市肿瘤医院病理科江西赣州 341000

关键词：

淋巴瘤骨髓侵犯骨髓细胞形态学免疫组化聚合酶链反应

出版年：

2024

DOI：