Effect of Ursolic Acid-farnesyl Pyrophosphate Synthase Crosslinked Nanoparticles on Apoptosis of Cervical Cancer HeLa Cells and Its Mechanism
Objective:Effect of ursolic acid-farnesyl pyrophosphate synthase crosslinked nanoparticles (FPP@UA NPs) on apoptosis of HeLa cells and its mechanism.Method:From January 2022 to December 2023,the study on FPP@UA NPs activity of HeLa cells in cervical cancer were carried out.HeLa cell lines were collected and nanoparticles UA NPs and FPP@UA NPs were prepared,and divided into control group and FPP@UA NPs nanoparticle carrier groups of 4 μmol/L,8 μmol/L and 16 μmol/L.Inductively coupled plasma emission spectrometer was used to detect the uptake of FPP@UA NPs by HeLa cells,the apoptosis of HeLa cells was detected by flow cytometry,and the proliferation of HeLa cells was detected by cell counting kit 8 (CCK-8).The effect of FPP@UA NPs on HeLa cell survival was determined by clonal formation method.Result:The number of nanoparticles in HeLa cells in 8 μmol/L group was higher than that in 4 μmol/L group,the difference was statistically significant (P<0.05).The number of nanoparticles in HeLa cells in 16 μmol/L group was higher than that in 8 μmol/L group,the difference was statistically significant (P<0.05).Compared with control group,HeLa cell apoptosis rate increased,proliferation rate decreased and number of clones decreased in 4 μmol/L group,the differences were statistically significant (P<0.05).Compared with 4 μmol/L group,the apoptosis rate increased,proliferation rate decreased and number of clones decreased in HeLa cells in 8 μmol/L group,the differences were statistically significant (P<0.05).Compared with the 8 μmol/L group,the apoptosis rate was increased,the proliferation rate was decreased,and the number of clones was decreased in HeLa cells in the 16 μmol/L group,the differences were statistically significant (P<0.05).Conclusion:FPP@UA NPs may have certain significance in promoting the apoptosis of cervical cancer cells and inhibiting their cloning and proliferation.
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