Levels of IL-6,MMP-8,TIMP-1 in gingival crevicular fluid of patients with chronic pulpitis and their relationship with pulp vitality
[Objective]To detect the levels of interleukin-6(IL-6),matrix metalloproteinase 8(MMP-8),and tissue inhibitor of matrix metalloproteinase 1(TIMP-1)in gingival crevicular fluid of patients with chronic pulpitis,and to analyze the relationships between them and pulp vitality.[Methods]A total of 106 patients with chronic gingivitis(observation group)and 106 healthy volunteers(control group)were selected.The levels of IL-6,MMP-8,and TIMP-1 in gingival crevicular fluid were detected and the differences were compared between the two groups.The levels of IL-6,MMP-8,and TIMP-1 in gingival crevicular fluid of patients with different clinical characteristics were compared in the observation group.The pulp vitality was measured using an electrical pulp vitality meter to compare the levels of IL-6,MMP-8,and TIMP-1 in gingival crevicular fluid of patients with different pulp vitality.Pearson method was used to analyze the correlation between the pulp electrical activity(EPT)value and the levels of IL-6,MMP-8 and TIMP-1 in gingival crevicular fluid in the observation group.[Results]The levels of IL-6 and MMP-8 in gingival crevicular fluid in the observation group were higher than those in the control group(61.28±8.11 ng/L vs.38.15±7.04 ng/L,33.04± 6.02 ng/mL vs.20.05±4.18 ng/mL)(P＜0.05),and the level of TIMP-1 was lower than that in the control group(1.35±0.30 ng/mL vs.2.04±0.41 ng/mL)(P＜0.05).In the observation group,the levels of IL-6 and MMP-8 in gingival crevicular fluid of patients with disease duration ≥12 months and with diabetes were higher than those of patients with disease duration＜12 months and without diabetes(P＜0.05),and the levels of TIMP-1 were lower than those of patients with disease duration＜12 months and without diabetes(P＜0.05).The levels of IL-6 and MMP-8 in gingival crevicular fluid of patients with moderate and severe pain were higher than those of mild pain(P＜0.05),and those of severe pain were higher than moderate pain(P＜0.05),while the trend of TIMP-1 levels was the opposite.The levels of IL-6 and MMP-8 in gingival crevicular fluid of patients with decreased pulp vitality and no vitality were higher than those of normal vitality(P＜0.05),and those of no vitality were higher than decreased pulp vitality(P＜0.05),while the trend of TIMP-1 levels was the opposite.The EPT of the affected teeth in the observation group was positively correlated with the levels of IL-6 and MMP-8 in gingival crevicular fluid(r=0.715,0.689,P＜0.05),and negatively correlated with the level of TIMP-1(r=-0.673,P＜0.05).[Conclusion]In patients with chronic gingivitis,the level of IL-6 and MMP-8 in gingival crevicular fluid increase,and the level of TIMP-1 decreases,which are related to the course of disease,the degree of pain,diabetes and pulp vitality.
万方数据
维普
