Correlation between expression and distribution of virulence genes of ESBLs in mucous lung and their drug resistance
[Objective]To analyze the expression,drug resistance and virulence gene distribution of ESBLs in mucous type klebsiella pneumoniae(MT-KPN).[Methods]A total of 725 non-repeatable KPN strains from March 2021 to March 2022 were collected from Zhengzhou First People's Hospital.Totally 123 MT-KPN strains were isolated by mucus wire test.The expression of ESBLs and drug resistance were analyzed by CITEK 2 Compact system,and the distribution of virulence genes were analyzed by PCR.[Results]The department with the highest detection rate of KPN was intensive care medicine.In the same department,KPN types were significantly different in intensive care,general practice and neurosurgery,while cKPN was detected only in nephrology department.The 123 MT-KPN strains included 34 ESBLs-positive strains and 89 ESBLs-negative strains.The drug resistance of ESBLs-positive bacteria was as high as 100.00％of 7 drugs(cefuroxime,cefotaxime,ceftazme,cefepime,cefoxime,amtrannan,amoxicillin/clavulanate),and the drug resistance of ESBLs-negative bacteria was significantly lower than that of ESBLs-positive bacteria(P＜0.05).The drug resistance of ESBLs-positive bacteria to tobramycin,imipenem,amikacin,piperacillin/tazobactam were higher than ESBLs-negative bacteria(P＜0.05).ESBLs-positive bacteria were more resistant to gentamicin,meropenem,ciprofloxacin and levofloxacin than ESBLs-negative bacteria(P＞0.05).wzyK2 gene detection rate of MT-KPN capsule serotype was the highest;there was no significant difference between ESBLs-positive bacteria and ESBLs-negative bacteria(P＞0.05);mrkD gene detection rate of MT-KPN virulence was the highest;aerobactin,iroN and wcaG detection rates of ESBLs-positive bacteria were lower than ESBLs-negative bacteria(P＜0.05);there was no significant difference between ESBLs-positive bacteria and ESBLs-negative bacteria in detection rate of other virulence genes(P＞0.05).[Conclusion]The detection rate of virulence genes carried by MT-KPN is high,and rational drug use should be combined with drug resistance and distribution of virulence genes.
万方数据
维普
