Correlation analysis of placental ABCB4 expression with blood indicators and pregnancy outcomes in intrahepatic cholestasis of pregnancy
[Objective]To analyze the correlation between the expression of ABCB4 gene in placental tissue and blood indicators and pregnancy outcomes in intrahepatic cholestasis of pregnancy(ICP)patients.[Methods]This article is a retrospective study,with cases included from January 2021 to December 2022.The study subjects were 112 ICP patients.According to different clinical pregnancy outcomes,60 ICP patients with adverse pregnancy outcomes were classified as the poor prognosis group,and 52 ICP patients with normal delivery were classified as the good prognosis group.The placental ABCB4 positive expression rate,ABCB4 mRNA relative expression level,serum bile acid,and liver function indicators of the two groups of patients were compared.The correlation between placental ABCB4 expression and blood indicators in ICP patients was verified by Spearman correlation coefficient.The predictive efficacy of ABCB4 expression in placenta for adverse pregnancy outcomes was verified by plotting ROC.[Results]After testing,the positive expression rate of ABCB4 in patients with poor prognosis was 85.00％(51/60),which was higher than 73.08％(38/52)in the group with good prognosis.The relative expression level of ABCB4 mRNA was 7.75±2.31,and lower than that in the group with good prognosis 9.03±2.49(P＜0.05).The bile acid levels of patients with poor prognosis were 55.25±10.31 mmol/L,AST levels were 88.39±10.47 U/L,and ALT levels were 85.11±10.61 U/L,which were higher than those of patients with good prognosis(48.24±10.47 mmol/L,80.24±10.31 U/L,78.45±10.17 U/L)(P＜0.05).According to Spearman correlation coefficient test,the positive expression rate of ABCB4 in the placenta continuously increases with the increase of bile acid,AST,and ALT levels,while the relative expression level of ABCB4 mRNA decreases.According to the ROC curve validation,the higher the positive expression rate of ABCB4 and the lower the relative expression level of ABCB4 mRNA,the higher the risk of adverse pregnancy outcomes in ICP patients.[Conclusion]The positive expression rate of ABCB4 in the placenta of ICP patients will significantly increase,and the relative expression level of ABCB4 mRNA will significantly decrease.The positive expression rate of ABCB4 is positively correlated with the patient's blood indicators and the incidence of adverse pregnancy outcomes,while the relative expression level of ABCB4 mRNA is negatively correlated with the patient's blood indicators and the incidence of adverse pregnancy outcomes.
ABCB4 expression in placentaintrahepatic cholestasis of pregnancyblood indicatorspregnancy outcomecorrelation analysis
万方数据
维普
