Role of Metrnl in regulation of endometrial receptivity and the association between its abnormal expression and repeated implantation failure
[Objective]To analyze the association between Metrnl expression and endometrial receptivity and repeated implantation failure(RIF)in infertility patients.[Methods]This article is a retrospective study,with cases included from October 2020 to December 2022.The study subjects were 113 infertile patients,all of whom were treated with in vitro fertilization/intracytoplasmic sperm injection and embryo transfer(IVF/ICSI-ET)for assisted reproductive technology(ART).Based on the results of ART treatment,60 patients with successful implantation were classified as the success group,and 53 patients with RIF were classified as the failure group.The endometrial receptivity related indicators and Metml expression of the two groups of patients were tested and compared.The correlation between Metrnl expression and endometrial receptivity was verified by Spearman correlation coefficient,and the predictive efficacy of Metrnl expression on RIF outcomes in infertile patients was verified by plotting receiver operating characteristic(ROC)curve and referencing the area under the curve(AUC).[Results]After testing,the endometrial thickness and volume of the failed group were 10.25±3.41 mm and 4.14±2.26 mL,respectively,which were lower than those of the successful group(12.33±3.24 mm and 5.77±2.38 mL).Endometrial blood flow resistance index(RI)of the failed group was 2.44±0.75,which was higher than that of the successful group(1.88±0.27)(P＜0.05).After testing,the expression level of PP14 was 23.44±5.28 μg/L,GAL-1 expression score was 4.15±1.26 points,and OPN molecular expression level was 38.24±10.46 kDa in the failed group,which were lower than those in the successful group(26.25±5.16 μg/L,5.44±1.76 points,and 45.33±10.37 kDa(P＜0.05).The expression level of Metrnl in the failed group was 95.25±20.61 pg/mL,which was higher than that in the successful group(78.24±20.16 pg/mL(P＜0.05).After Spearman correlation coefficient test,the expression of Metrnl was negatively correlated with endometrial thickness,endometrial volume,PP14,GAL-1,OPN,and positively correlated with RI.The ROC curve showed that the sensitivity,specificity,cut-off value and AUC value of Metml high expression for predicting RIF in infertile patients were 80.25％,78.33％,90.45 pg/mL and 0.855 respectively.[Conclusion]The expression level of Metrnl is closely related to endometrial receptivity,and high expression of Metrnl is a risk factor for RIF outcomes in infertile patients.When Metrnl expression is not lower than 90.27 pg/mL,infertile patients are more likely to develop RIF.
infertilityrepeated planting failureNickel like secretory proteinendometrial receptivitycorrelation analysis
万方数据
维普
