Screening of reference genes in different human-derived cells
[Objective]To screen internal reference genes that can be stably expressed in different human-derived cells,in order to provide a certain reference for quantitative research of different tissues.[Methods]The Expression of β-Globin,GAPDH,18S rRNA and Rnase P four candidate reference genes in 36 types of human-derived cells was detected by real-time fluorescence quantitative PCR(RT-qPCR).The stability of four candidate reference genes was evaluated using geNorm,NormFider and BestKeeper software.[Results]In the same type of cell,the expression of Rnase P is relatively high;in different cells,the expression of 18S rRNA is relatively stable.[Conclusion]Comprehensive analysis found that although the candidate reference gene 18S rRNA is relatively stable,it is not the best choice.
万方数据
维普
