Relationship between expression levels of EPCR and PECAM-1 in brain tissues of patients with cerebral hemorrhage and severity of disease
[Objective]To explore the relationship between the expression levels of endothelial cell protein C receptor(EPCR)and platelet endothelial cell adhesion molecule 1(PECAM-1)in brain tissue of patients with cerebral hemorrhage and the severity of the disease.[Methods]One-hundred patients with cerebral hemorrhage who were treated in Zhumadian Central Hospital from March 2019 to August 2020 were selected as the research objects.The 0.5 cm brain tissue near the hematoma(intracerebral hemorrhage tissue specimen,cerebral hemorrhage group)and brain tissue at the location of distant hematoma(control tissue specimen,control group)were collected.According to the amount of bleeding,the patients were divided into minor group(n=35),medium group(n=44)and massive group(n=21);according to the severity of the disease,patients were divided into mild group(n-40),moderate group(n=40)cases and severe group(n=20).The expression levels of EPCR and PECAM-1 in tissues were detected with immunohistochemistry.The correlation between the positive expression rates of EPCR and PECAM-1 in cerebral hemorrhage tissue and National Institutes of Health Stroke Scale(NIHSS)score was analyzed with Spearman method.The influencing factors of cerebral hemorrhage were analyzed with logistic regression.[Results]The results of immunohistochemical staining showed that the positive expression rate of EPCR in the cerebral hemorrhage group was significantly higher than that in the control group,while the positive expression rate of PECAM-1 in the cerebral hemorrhage group was significantly lower than that in the control group,and the differences were statistically significant(P＜0.05).The positive expression rate of EPCR in cerebral hemorrhage tissues of minor group,medium group and massive group increased successively,and the positive expression rate of PECAM-1 decreased successively(P＜0.05).The positive expression rate of EPCR in cerebral hemorrhage tissues in the mild group,moderate group and severe group increased successively,and the positive expression rate of PECAM-1 decreased successively(P＜0.05).The positive expression rate of EPCR in cerebral hemorrhage tissue was positively correlated with the patient's NIHSS score(r=0.416,P＜0.001),and the positive expression rate of PECAM-1 was negatively correlated with the patient's NIHSS score(r=-0.337,P＜0.001).EPCR was a risk factor affecting the occurrence of cerebral hemorrhage,and PECAM-1 was a protective factor affecting the occurrence of cerebral hemorrhage(P＜0.05).[Conclusion]EPCR is highly expressed in brain tissues of patients with cerebral hemorrhage,while PECAM-1 is lowly expressed.Both of them are related to the severity of the disease and the amount of bleeding,and may be used as potential markers for evaluating the condition of patients with cerebral hemorrhage.
endothelial cell protein C receptorplatelet endothelial cell adhesion molecule 1cerebral hemorrhagedisease severity
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