Single-cell transcriptomic bioinformatics study of potential mechanisms of T cells involvement in chronic obstructive pulmonary disease
[Objective]To explore the potential mechanism of T cell involvement in the pathogenesis of chronic obstructive pulmonary disease (COPD) using single-cell transcriptome bioinformatics methods.[Methods]Single-cell transcriptome dataset GSE173896 was downloaded from the Gene Expression Omnibus database,the data were filtered and normalized using the Seurat package in R.Cells with similar characteristic genes were clustered and annotated to screen for cell subtypes associated with the development of COPD.T cell subtypes were extracted for secondary analysis.Cellchat package in R was used to analyze cell communication between T cells and other cell subtypes.Differential expression genes in T cells were screened and subjected to enrichment analysis to construct a protein interaction network to identify core genes.Three datasets (GSE69818,GSE37768,and GSE106986) were downloaded to verify and screen key core genes related to the development of COPD,and receiver operating characteristic (ROC) curves were plotted to evaluate the value of predicting the occurrence of COPD.[Results]A total of 9,313 lung tissue cells from patients with COPD and 11,716 lung tissue cells from healthy controls were filtered.T cells accounted for a higher proportion of lung tissue cells in patients with COPD,and the number of helper T cells was higher than that in the healthy control group,while the number of regulatory T cells was lower.The interaction intensity between T cells and monocytes,B cells,endothelial cells,and natural killer cells was significantly upregulated.A total of 46 upregulated and 32 downregulated differential expression genes were obtained,which were significantly enriched in signaling pathways such as interleukin-17,tumor necrosis factor-α,nuclear factor kappa B,and Toll-like receptor.TIMP1 and CXCL5 were identified as key core genes related to the development of COPD.[Conclusion]There are significant differences in the distribution of lung tissue cells between COPD and healthy individuals.T cells are closely related to the pathogenesis of COPD and may be potential target cells for prevention and treatment of COPD.The development of COPD involves multiple inflammatory signaling pathways.TIMP1 and CXCL5 are key core genes related to the development of COPD.
万方数据
维普
