Predictive value of egg count combined with sperm DNA fragment index and semen parameters for R-ICSI
[Objective]To explore the influencing factors of fertilization failure during short-term in vitro fertilization (IVF),in order to provide a basis for selecting appropriate pregnancy assistance methods.[Methods]The IVF cycle data of short-term fertilization at the Reproductive Center of Women and Children Healthcare Hospital of Zhuzhou from January 2021 to September 2023 were retrospectively analyzed.According to the fertilization results,the patients were divided into the rescue intra cytoplasmic sperm injection (R-ICSI) group (n=49) and the short-term IVF group (n=402).The data of infertile couples were compared,including age,type of infertility,duration of infertility,number of Gn days,total Gn count,number of eggs obtained,female body mass index (BMI),follicle-stimulating hormone (FSH),anti-Müllerian hormone (AMH),semen volume,sperm concentration,forward movement (PR grade) sperm,normal sperm morphology and sperm DNA fragmentation index (DFI).[Results]There were significant differences in egg count,sperm concentration,normal sperm morphology,DFI between the R-ICSI group and the short-term IVF group (P<0.05),while there were no significant differences in age,infertility type,infertility years,Gn days,Gn total,female BMI,FSH,AMH,semen volume,PR grade sperm between the two groups (P>0.05).The proportion of eggs obtained in the R-ICSI group was significantly different from that in the short-term IVF group,with a count of<5 and ≥30 eggs.At the same time,the proportion of mild oligospermia and moderate dysspermia was also significantly higher than that in the short-term IVF group (P<0.05).[Conclusion]When the number of eggs obtained is<5 or ≥30,abnormal sperm concentration,normal sperm morphology,and abnormal DFI are encountered,especially when these indicators are abnormal at the same time,indicating a higher probability of fertilization disorders in patients.R-ICSI can be implemented as soon as possible to improve egg utilization rate.
rescue intra cytoplasmic sperm injectionnumber of retrieved oocytessperm concentrationnormal sperm morphologysperm DNA fragmentation index
万方数据
维普
