摘要
目的 分析中枢性性早熟(CPP)女童肠道菌群的多样性、结构及功能特征,挖掘其与同年龄段健康女童的差异菌群及潜在功能.方法 选择2023年11～12月在湖北省中医院儿科门诊就诊并确诊为CPP的女童20例为CPP组,同期选取同年龄段健康女童志愿者20例为对照组.收集两组粪便样本进行16S rRNA测序,分析两组肠道菌群组成结构及功能差异.结果 两组肠道菌群的a多样性比较差异无统计学意义(P＞0.05).CPP组肠道菌群的β多样性显示出明显的分离趋势,两组比较差异有统计学意义(P＜0.05).CPP组的基因富含内分泌和代谢疾病、核苷酸代谢、氨基酸代谢、细胞加工及信号转导等功能.结论 CPP女童与健康女童在肠道菌群数目、种类多样性、分布均匀性方面差异不大,但在结构上存在显著差异;预测内分泌和代谢疾病、核苷酸代谢、氨基酸代谢、细胞加工及信号转导等功能与CPP有潜在相关性.
Abstract
Objective To analyze the diversity,structure and functional characteristics of the intestinal flora of girls with central precocious puberty(CPP),and to explore the differential flora and potential functions of them with those of healthy girls of the same age.Methods We selected 20 girls who attended the pediatric outpatient clinic of Hubei Provincial Hospital of Traditional Chinese Medicine from November to December 2023 and were diagnosed with CPP as the CPP group,and 20 healthy girls volunteers of the same age during the same period were included as the control group.Stool samples were collected for 16S rRNA sequencing to analyze the differences in the composition,structure and function of the intestinal flora between the two groups.Results The difference in a-diversity of intestinal flora between the two groups was not statistically significant(P＞0.05).The β-diversity of intestinal flora in the CPP group showed a clear tendency to segregate,and the difference between the two groups was statistically significant(P＜0.05).Genes in the CPP group were enriched for endocrine and metabolic disorders,nucleotide metabolism,amino acid metabolism,cellular processing,and signal transduction.Conclusion Differences between girls with CPP and healthy girls are not significant in terms of the number,species diversity,and distributional homogeneity of intestinal flora,but there are significant structural differences;the functions in predicting endocrine and metabolic diseases,nucleotide metabolism,amino acid metabolism,cellular processing,and signaling are potentially correlated with CPP.
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