Effects of Baibo Pills and its Modified Prescriptions on CD8+T Cells and Melanocytes in Vitiligo Mice
Objective To explore the effect of Baibo pills and its modified prescriptions on CD8+T cells and melanocytes in vitiligo mice.Methods Normal C57BL/6 mice were used as the blank group,and the mice of same strain were randomly divided into one model group,one positive drug group,and one Baibo pills and its modified prescription groups.Except for the blank group,all other groups constructed vitiligo animal models with autoreactive CD8+T cells targeting skin melanocytes.After successful modeling,tofacitinib,Baibo pills and its modified prescriptions were administered by gavage,while the blank group and model group were given equal amounts of pure water.All mice were administered by gavage for 10 weeks.Using a small studio and digital camera to capture the tail skin of mice,and observing the depigmentation of skin lesions after Image J image processing.Whole mount immunofluorescence was used to observe the number and distribution of CD8+T cells and melanocytes.Flow cytometry was used to observe the proportion and function of CD8+T cells and melanocytes.Results Compared with the blank group,the model group showed an increase in the depigmentation area of tail lesions,infiltration of CD8+T cells,and loss of melanocytes.The proportion of CD3+CD8+T,CD8+CD69+T,and CD8+CXCR3+T cells increased,while the proportion of CD45-CD117+melanocytes decreased.Compared with the model group,Baibo pills and its modified prescriptions could improve the depigmentation,reduce CD8+T cell infiltration and melanocyte deficiency,decrease the proportion of CD3+CD8+T,CD8+CD69+T,CD8+CXCR3+T cells,and increase the proportion of CD45-CD117+melanocytes to varying degrees.The effect of Baibo pills was the most significant,while the effect of Baibo Pills without nourishing blood,activating blood,and unblocking meridian herbs was the least significant.Conclusion Baibo pills and its modified prescriptions could inhibit the number and function of CD8+T cells,protect melanocytes,and reduce the depigmentation of tail skin lesions in vitiligo mice to varying degrees.Nourishing blood,activating blood,and unblocking meridian herbs may play a core role.
万方数据
