PI3K/AKT信号通路与乳腺病变弹性特征的相关性
Correlation Between PI3K/AKT Signaling Pathway and Elastic Characteristics of Breast Lesions
姜莹 1王博 2李诗雨 2牛瑞兰 2李俊康 2王知力1
作者信息
- 1. 南开大学医学院,天津 300071;解放军总医院第一医学中心超声诊断科,北京 100853
- 2. 解放军总医院第一医学中心超声诊断科,北京 100853
- 折叠
摘要
目的 探讨磷脂酰肌醇-3-激酶/丝苏氨酸蛋白激酶(PI3K/AKT)信号通路与乳腺病变弹性特征的相关性.资料与方法前瞻性分析 2021 年 5 月—2022 年 5 月于解放军总医院接受手术的 114 例患者共 115 个乳腺病变,术前行超声和剪切波弹性成像检查.采用免疫组化染色检测组织标本中PI3K/AKT蛋白水平的表达,分析染色结果与剪切波弹性成像最大弹性模量值、平均弹性模量值、最小弹性模量值、病变与周围组织弹性比值的相关性.结果 115 个乳腺病变中,手术病理显示乳腺良性病变50个,恶性病变65个(25个伴腋窝淋巴结转移).良性病变与恶性病变不伴腋窝淋巴结转移、恶性病变伴腋窝淋巴结转移的最大弹性模量值(F=40.47)、平均弹性模量值(F=45.11)、病变与周围组织弹性比值(F=48.98)、"硬环征"检出率(χ2=62.25)、PI3K/p-PI3K表达水平(F=15.19、58.95)及AKT/p-AKT表达水平(F=46.94、74.21)比较,差异均有统计学意义(P<0.05).PI3K/p-PI3K及AKT/p-AKT表达水平与最大弹性模量值、平均弹性模量值、病变与周围组织弹性比值均呈正相关(r=0.475、0.475、0.451;r=0.533、0.540、0.542;r=0.371、0.402、0.445;r=0.482、0.455、0.545,P均<0.05).结论 乳腺病变的PI3K/AKT表达水平与弹性特征具有相关性,提示其在乳腺病变弹性特征调控中发挥重要作用.
Abstract
Purpose To explore the correlation between phosphatidylinositol 3 kinase/serine-threonine kinase(PI3K/AKT)signaling pathway and elastic characteristics of breast lesions.Materials and Methods A total of 115 breast lesions were prospectively analyzed in 114 patients who underwent surgery from May 2021 to May 2022 at Chinese PLA General Hospital.Ultrasound and shear wave elastography were performed preoperatively.Immunohistochemical staining was used to detect the expression of PI3K/AKT protein levels in the tissue specimens,and the correlation between the staining results and the elastic parameters of shear wave elastography was analyzed.Results Surgical pathology revealed benign breast lesions in 50 cases and malignant lesions in 65 cases(25 cases with axillary lymph node metastasis).The maximum modulus of elasticity(F=40.47),the average modulus of elasticity(F=45.11),the ratio of elasticity of the lesion to that of the surrounding tissue(F=48.98),the detection rate of"hard ring sign"(χ2=62.25),the expression level of PI3K/p-PI3K(F=15.19,58.95)and AKT/p-AKT(F=46.94,74.21)were found in benign and malignant lesions without axillary lymph node metastasis,malignant lesions with axillary lymph node metastasis(all P<0.05).The expression levels of PI3K/p-PI3K and AKT/p-AKT were positively correlated with the maximum elastic modulus value,the mean elastic modulus value,and the ratio of elasticity of the lesion to the surrounding tissues(r=0.475,0.475,0.451;r=0.533,0.540,0.542;r=0.371,0.402,0.445;r=0.482,0.455,0.545,all P<0.05).Conclusion The expression level of PI3K/AKT in breast lesions is correlated with elastic characteristics,suggesting that it plays an important role in the regulation of elastic characteristics of breast lesions.
关键词
乳腺肿瘤/超声检查/弹性显像技术/淋巴转移/信号传导/病理学,外科Key words
Breast neoplasms/Ultrasonography/Elastography/Lymphatic metastases/Signaling/Pathology,surgical引用本文复制引用
基金项目
国家自然科学基金(82071925)
军队保健课题(22BJZ23)
装备综合研究项目(LB20211A010011)
出版年
2024
NETL
NSTL
万方数据