Purpose To prepare multifunctional SiO2@MnO2 nanoplatforms(SiO2@MnO2 NPs)with chemo dynamic therapy(CDT)efficacy and observe their effects on in vitro MRI and ultrasound imaging.Materials and Methods SiO2 nanoparticles(SiO2 NPs)were firstly prepared by dehydration-condensation reaction of ethyl orthosilicate in alcohol phase,and then SiO2@MnO2 NPs were prepared by reduction reaction of polyethylene glyco with KMnO4.The morphology of the two nanoparticles was observed,and the particle size and surface potential were detected.Methylene blue degradation verified the ability of SiO2@MnO2 NPs to produce toxic hydroxyl radicals,one of the reactive oxygen species.Simulation of different conditions to evaluate the effect of nanoparticles for in vitro MRI imaging.To observe the cellular uptake and the intracellular production of reactive oxygen species by SiO2@MnO2 NPs.CDT efficacy of nanoparticles was assessed on human pancreatic cancer cell PANC-1 cells.Results The SiO2@MnO2 NPs was successfully prepared,and the shell of fragmented MnO2 was observed to be encapsulated around the surface of spherical SiO2 NPs under transmission electron microscopy.The average size was(115.9±2.0)nm and the average zeta potential was(-32.51±0.30)mV.Methylene blue was gradually degraded with the increased concentration of SiO2@MnO2 NPs,suggesting the generation of toxic hydroxyl radicals.In vitro simulated tumor microenvironment,SiO2@MnO2 NPs could enhance the MRI and ultrasound imaging effect.Large amount of reactive oxygen species production was detected in PANC-1 cells and exerted CDT effect to kill tumor cells.Conclusion The successfully prepared multifunctional SiO2@MnO2 NPs possess favorable CDT effect and dual-mode imaging effect,which can also kill PANC-1 cells,laying a foundation for synergistic CDT and the construction of multifunctional nanoplatform.
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