首页|基于7.0T心血管磁共振探究苍艾挥发油对高原心功能损伤大鼠的治疗作用

基于7.0T心血管磁共振探究苍艾挥发油对高原心功能损伤大鼠的治疗作用

Therapeutic Effect of Cang-ai Volatile Oil on High Altitude Rats with Cardiac Impairment Based on 7.0T Cardiovascular Magnetic Resonance

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目的 基于7.0T心血管磁共振(CMR)探索苍艾挥发油(CAVO)对青藏高原低压低氧环境下心功能损伤模型大鼠的心脏保护作用.材料与方法 将40只SD大鼠随机分为正常组、高原模型组、CAVO治疗组和红景天治疗组,每组10只.除正常组外,其余各组大鼠从平原(海拔500m)转运至青藏高原(海拔4 250 m)饲养2个月后,使用相应药物灌胃给药14 d.使用7.0T高场强CMR测量左心室功能,并采用组织追踪技术分析心肌应变.使用HE染色观察心肌细胞形态,Masson染色观察心肌间质纤维化,小麦胚芽凝集素染色观察心肌细胞肥大情况,透射电镜观察各组线粒体形态学改变.检测血清中肌酸激酶、肌酸激酶同工酶、乳酸脱氢酶、心肌肌钙蛋白T、超氧化物歧化酶、丙二醛和谷胱甘肽过氧化物酶水平.使用流式细胞术检测细胞内活性氧水平.结果 CAVO治疗组大鼠左心室射血分数高于高原模型组[(66.61±1.38)％比(60.94±3.21)％;t=3.969,P=0.032)],左心室整体环向应变高于高原模型组(-25.68±1.30比-22.84±1.17;t=3.967,P=0.003);HE、Masson和小麦胚芽凝集素染色显示高原模型组心肌细胞出现肥大、坏死以及间质纤维化,超微结构破坏,CAVO治疗后有所改善.CAVO治疗组大鼠血清中心肌肌钙蛋白 T 水平比高原模型组明显下降[(314.03±20.05)pg/ml 比(518.30±18.13)pg/ml;t=13.090,P=).001].结论 CAVO治疗可减轻高原低压缺氧引起的心脏损伤,其效果可通过7.0T高场强CMR动态、无创性检测.
Purpose To explore the cardioprotective effect of cang-ai volatile oil(CAVO)on rats with cardiac function impairment model under low-pressure and low-oxygen environment in Tibet Plateau based on 7.0T cardiovascular magnetic resonance(CMR)imaging.Materials and Methods Forty SD rats were randomly divided into the normal group,the high altitude model group,the CAVO-treated group and the rhodiola rosea-treated group,with 10 rats in each group.Except for the normal group,the rats in other groups were transferred from the plain(500 m above sea level)to the Tibet Plateau(4 250 m above sea level)for two months,and then administered with the corresponding drugs by gavage for 14 d.The left ventricle function was measured by using a 7.0T high-field strength CMR and myocardial strain was analysed by using tissue tracing technique.HE staining was used to observe the morphology of cardiomyocytes,Masson staining to observe interstitial fibrosis,wheat germ agglutinin staining to observe cardiomyocyte hypertrophy,and transmission electron microscopy to observe the morphological changes of mitochondria in each group.Serum levels of creatine kinase,creatine kinase isoenzyme,lactate dehydrogenase,cardiac troponin T,superoxide dismutase,malondialdehyde and glutathione peroxidase were detected.Intracellular reactive oxygen species levels were detected using flow cytometry.Results The left ventricular ejection fraction of rats in the CAVO-treated group was higher than that of the high altitude model group[(66.61±1.38)％vs.(60.94±3.21)％;t=3.969,P=0.032];meanwhile,the global circumferential strain of the left ventricle in the CAVO-treated group was higher than that of the high altitude model group(-25.68±1.30 vs.-22.84±1.17;t=3.967,P=0.003).HE,Masson and wheat germ agglutinin staining showed hypertrophy and necrosis as well as interstitial fibrosis and ultrastructural disruption of cardiomyocytes in the high altitude model group,which improved after CAVO treatment.The level of cardiac troponin T in the serum of rats with CAVO treatment group was significantly decreased compared with that of the high altitude model group[(314.03±20.05)pg/ml vs.(518.30±18.13)pg/ml;1=13.090,P=0.001].Conclusion CAVO treatment can reduce cardiac injury caused by low-pressure hypoxia in high altitude,and its effect can be detected dynamically and non-invasively by 7.0T high-field strength CMR.

Cang-ai volatile oilAltitude sicknessMagnetic resonance imagingMyocardial strainVentricular function,leftRats,Sprague-DawleyAnimal experimentation

梁博深、陈柏君、尹红科、王磊、陈皓田、方鑫、李亥辰、索靖航、熊磊、张永海、郜发宝

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云南中医药大学,云南 昆明 650500

四川大学华西医院放射科,四川成都 610041

成都中医药大学附属医院放射科,四川 成都 610041

嘉兴大学,浙江嘉兴 314001

青海省第五人民医院,青海西宁 810007

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苍艾挥发油 高空病 磁共振成像 心肌应变 心室功能,左 大鼠,Sprague-Dawley 动物实验

国家自然科学基金云南省专家工作站四川省自然科学基金

81930046202105AF1500372023NSFSC1717

2024

10.3969/j.issn.1005-5185.2024.09.001

中国医学影像学杂志
中国医学影像技术研究会

中国医学影像学杂志

CSTPCD北大核心
影响因子：1.37
ISSN：1005-5185
年,卷(期)：2024.32(9)
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