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高效液相色谱串联质谱法测定血清硫化氢方法的建立及评价

Establishment and evaluation of HPLC-MS/MS method in determining serum H2S

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 维普
  • 万方数据
目的:建立高效液相色谱串联质谱法(HPLC-MS/MS)对于血清中硫化氢(H2S)含量的测定方法,对血清中H2S进行测定.方法:收集2023年4-5月在首都医科大学附属北京积水潭医院检测的30名受检者血清学样本,根据检测结果是否存在骨质疏松将其分别纳入骨质疏松组和对照组,每组15例.分别使用HPLC-MS/MS及酶标法进行血清中H2S测定,评价两种方法测定结果的精密度、准确度及相关性.结果:HPLC-MS/MS通过检测血清中的亚甲基蓝,可快速检测血清中H2S的含量,分析时间仅为1.5 min,特异性比较高,实测得质控血浆相对标准偏差(RSD)值为8.77%,质控血浆加标RSD值为4.58%,纯水加标RSD值为8.23%,且精密度均符合定量检测要求(＜20%).用上述数据测定加标回收率为103.5%,准确度符合定量检测要求(回收率为103.5%).结论:HPLC-MS/MS检测H2S快捷并且准确,能够准确地检测出血清中H2S的含量,该方法具有快捷、通量高、灵敏度高且稳定性好的优点,有助于更好的展开血清中H2S含量在人体细胞通路中的基础研究.
Objective:To establish a determination method of high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)for serum hydrogen sulfide(H2S),so as to determine serum H2S.Methods:This study collected serum samples of 30 patients who admitted to Beijing Jishuitan Hospital affiliated to Capital Medical University from April 2023 to May 2023,and they were divided into osteoporosis group and control group according to whether existed osteoporosis,with 15 cases in each group.HPLC-MS/MS and enzyme-linked immunosorbent assay were used respectively to determine serum H2S.And then,the precision,accuracy and correlation between the two methods were evaluated.Results:HPLC-MS/MS can fast detect the content of serum H2S through detecting methylene blue in the serum,which analysis time was only 1.5 minutes,and its specificity was higher.The relative standard deviation(RSD)value of quality control plasma was 8.77%,and that of quality control plasma with the standard and pure water with standard were respectively 4.58% and 8.23%.The precisions of them met the requirement of detection(less than 20%).The recovery was 103.5% through used the above data,and the accuracy accorded with the requirements of quantitative detection(recovery was 103.5%).Conclusion:HPLC-MS/MS method is rapid and accurate in detecting H2S,which can accurately detect the content of serum H2S.This method has a series of advantages include fast,high throughput,high sensitivity and favorable stability,which contributes to conduct basic research of the content of serum H2S in the cellular pathways of human.

High performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)Hydrogen sulfideOsteoporosisMethylene blue

孟祥宇、张翱、李春艳

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首都医科大学附属北京积水潭医院中心实验室 北京 100035

首都医科大学附属北京积水潭医院检验科 北京 100035

高效液相色谱串联质谱法(HPLC-MS/MS) 硫化氢(H2S) 骨质疏松 亚甲基蓝

北京市创伤骨科研究所改革与发展专项青年项目北京市创伤骨科研究所改革与发展专项平台建设项目北京积水潭医院自然科学基金培育计划项目

QN-2023-13PT-2023-01ZR-202205

2024

10.3969/j.issn.1672-8270.2024.02.009

中国医学装备
中国医学装备协会

中国医学装备

CSTPCD
影响因子：0.882
ISSN：1672-8270
年,卷(期)：2024.21(2)
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