超声弹性成像联合血清miR-26b-5p及环氧化酶-2检测对早期子宫肌瘤的临床诊断价值
Clinically diagnostic value of ultrasound elastography combined with serum miR-26b-5p and COX-2 examination for early hysteromyoma
郭晓波 1陈金玉 1檀融融1
作者信息
- 1. 无锡联勤保障中心第九〇〇医院超声科 福州 350002
- 折叠
摘要
目的:探讨超声弹性成像联合血清微小RNA-26b-5p(miR-26b-5p)、环氧化酶-2(COX-2)检测对早期子宫肌瘤的诊断价值.方法:选取2020年10月至2022年9月在无锡联勤保障中心第九〇〇医院诊断的228例疑似子宫肌瘤患者,以病理切片检查结果为"金标准",将其分为阳性组(124例)和阴性组(104例),两组均行超声弹性成像检查,采用实时荧光定量聚合酶链反应(PCR)法检测患者血清miR-26b-5p表达水平,酶联免疫吸附法检测血清COX-2水平,通过受试者工作特征(ROC)曲线下面积(AUC),分析血清miR-26b-5p、COX-2对子宫肌瘤的诊断价值,采用四表格法分析超声弹性成像以及超声弹性成像联合血清miR-26b-5p、COX-2对子宫肌瘤的诊断价值.结果:经病理切片检查,228例患者中子宫肌瘤阳性124例,阴性104例;超声弹性成像结果显示阳性117例,阴性111例,超声弹性成像检查诊断的灵敏度为74.19%,特异度为75.96%,准确率为75.00%.阳性组患者血清miR-26b-5p水平明显低于阴性组,COX-2水平明显高于阴性组,两组比较差异有统计学意义(t=4.519、5.601,P＜0.05).血清miR-26b-5p诊断子宫肌瘤的AUC为0.749,灵敏度为95.97%,特异度为46.15%,最佳截断值为1.10;血清COX-2诊断子宫肌瘤的AUC为0.835,灵敏度为66.13%,特异度为84.62%,最佳截断值为40.58 mg/L;超声弹性成像联合血清miR-26b-5p、COX-2诊断的灵敏度为93.55%,特异度为86.54%,准确率为90.35%,与单独诊断比较差异有统计学意义(x2=23.158、17.169,P＜0.05).结论:超声弹性成像联合血清miR-26b-5p、COX-2诊断早期子宫肌瘤具有较高的效能.
Abstract
Objective:To investigate the diagnostic value of ultrasound elastography combined with serum microRNA-26b-5p(miR-26b-5p)and cyclooxygenase-2(COX-2)detection for early hysteromyoma.Methods:A total of 228 patients with suspected early hysteromyoma who were diagnosed in the 90th Hospital of the Joint Service Support Center from October 2020 to September 2022 were selected as the observation objects.Based on the results of pathological section examination as the"gold standard",all subjects were divided into a positive group(124 cases)and a negative group(104 cases),and ultrasound elasticity imaging examination was performed in both groups.The expression level of miR-26b-5p in serum was detected by real-time fluorescent quantitative polymerase chain reaction(Rt-PCR),and the level of serum COX-2 was detected by enzyme-linked immunosorbent assay(ELISA),and receiver operating characteristic(ROC)curve was applied to analyze the diagnostic values of serum miR-26b-5p and COX-2 for hysteromyoma.The four tables were applied to analyze the diagnostic values of ultrasound elastography and the combination of ultrasound elastography,serum miR-26b-5p and COX-2 for hysteromyoma.Results:The results of pathological examination indicated that 124 cases of 228 patients were positive result of hysteromyoma and 104 cases were negative result.The results of ultrasound elastography showed that 117 cases were positive,and 111 cases were negative,and the diagnostic sensitivity,specificity and accuracy of ultrasound elastography detection were respectively 74.19%,75.96%and 75.00%.Serum miR-26b-5p level of positive group was significantly lower than that of negative group,while the COX-2 level of positive group was significantly higher than that of negative group,and the differences of them between the two groups were statistically significant(t=4.519,5.601,P<0.05),respectively.The area under curve(AUC)value of ROC curve,sensitivity,specificity and the best cut-off value of serum miR-26b-5p were respectively 0.749,95.97%,46.15%and 1.10 in diagnosing hysteromyoma.The above indicators of serum COX-2 were respectively 0.835,66.13%,84.62%and 40.58 mg/L in diagnosing hysteromyoma.The sensitivity,specificity and accuracy of ultrasound elastography combined with serum miR-26b-5p and COX-2 were respectively 93.55%,86.54%and 90.35%,the differences were statistically significant(x2=23.158,17.169,P<0.05),respectively.Conclusion:Ultrasound elastography combined with serum miR-26b-5p and COX-2 has higher effectiveness in diagnosing the early hysteromyoma.
关键词
早期子宫肌瘤/超声弹性成像/微小RNA-26b-5p(miR-26b-5p)/环氧化酶-2(COX-2)/诊断Key words
Early hysteromyoma/Ultrasound elastography/microRNA-26b-5p(miR-26b-5p)/Cyclooxygenase-2(COX-2)/Diagnosis引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据